Defective Interfering Particle Generated by Internal Deletion of the Vesicular Stomatitis Virus Genome

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1980 Feb 1

PMID 6251252

Citations 23

Authors

D A Epstein

R C HERMAN

I Chien

R A Lazzarini

Affiliations

Soon will be listed here.

Abstract

The genome structure of the long, truncated defective interfering particle derived from the heat-resistant strain of vesicular stomatitis virus has been examined. Stocks of this defective interfering particle are shown to contain several different species having information primarily from the 3' half of the vesicular stomatitis virus genome; the proportions of these components vary depending on the passage history of the stock. The two most abundant types have been identified and characterized. One has complementary 5' and 3' termini and consequently appears as a circular molecule when examined by electron microscopy. The other cannot circularize and remains linear. The circular forms are consistently 8 to 10% longer than the linear molecules. Rapid sequencing analyses reveal that both forms retain the 5' parental viral terminal sequence, but only the linear form retains the parental 3'-terminal sequence which is the complement of the 5' end. Hybridization experiments and electron microscopic analyses indicate that the linear form has retained 320 to 350 nucleotides of the 5' parental sequence and was probably generated by an internal deletion of the vesicular stomatitis virus genome.

Citing Articles

An overview of process intensification and thermo stabilization for upscaling of Peste des petits ruminants vaccines in view of global control and eradication.

Bora M, Yousuf R, Dhar P, Singh R Virusdisease. 2018; 29(3):285-296.

PMID: 30159362 PMC: 6111956. DOI: 10.1007/s13337-018-0455-3.

Neuroanatomy goes viral!.

Nassi J, Cepko C, Born R, Beier K Front Neuroanat. 2015; 9:80.

PMID: 26190977 PMC: 4486834. DOI: 10.3389/fnana.2015.00080.

Quantitative characterization of defective virus emergence by deep sequencing.

Timm C, Akpinar F, Yin J J Virol. 2013; 88(5):2623-32.

PMID: 24352442 PMC: 3958049. DOI: 10.1128/JVI.02675-13.

Separation and sequence of the 3' termini of M double-stranded RNA from killer yeast.

Thiele D, Wang R, Leibowitz M Nucleic Acids Res. 1982; 10(5):1661-78.

PMID: 7041093 PMC: 320557. DOI: 10.1093/nar/10.5.1661.

Complete sequence analyses show that two defective interfering influenza viral RNAs contain a single internal deletion of a polymerase gene.

Nayak D, Sivasubramanian N, Davis A, Cortini R, Sung J Proc Natl Acad Sci U S A. 1982; 79(7):2216-20.

PMID: 6954536 PMC: 346162. DOI: 10.1073/pnas.79.7.2216.

References

Adler R, Banerjee A . Analysis of the RNA species isolated from defective particles of vesicular stomatitis virus. J Gen Virol. 1976; 33(1):51-60. DOI: 10.1099/0022-1317-33-1-51. View

Moss B . Utilization of the guanylyltransferase and methyltransferases of vaccinia virus to modify and identify the 5'-terminals of heterologous RNA species. Biochem Biophys Res Commun. 1977; 74(2):374-83. DOI: 10.1016/0006-291x(77)90314-x. View

Colonno R, Banerjee A . Mapping and initiation studies on the leader RNA of vesicular stomatitis virus. Virology. 1977; 77(1):260-8. DOI: 10.1016/0042-6822(77)90423-8. View

Meyer J, Neuwald P, Lai S, Maizel Jr J, Westphal H . Electron microscopy of late adenovirus type 2 mRNA hybridized to double-stranded viral DNA. J Virol. 1977; 21(3):1010-8. PMC: 515641. DOI: 10.1128/JVI.21.3.1010-1018.1977. View

Chow J, Schnitzlein W, REICHMANN M . Expression of genetic information contained in the RNA of a defective interfering particle of vesicular stomatitis virus. Virology. 1977; 77(2):579-88. DOI: 10.1016/0042-6822(77)90483-4. View

Johnson L, Lazzarini R . The 5' terminal nucleotide of RNA from vesicular stomatitis virus defective interfering particles. Virology. 1977; 77(2):863-6. DOI: 10.1016/0042-6822(77)90508-6. View

DINGMAN C, Peacock A . Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis. Biochemistry. 1968; 7(2):659-68. DOI: 10.1021/bi00842a022. View

Prevec L, Kang C . Homotypic and heterotypic interference by defective particles of vesicular stomatitis virus. Nature. 1970; 228(5266):25-7. DOI: 10.1038/228025a0. View

Petric M, Prevec L . Vesicular stomatitis virus--a new interfering particle, intracellular structures, and virus-specific RNA. Virology. 1970; 41(4):615-30. DOI: 10.1016/0042-6822(70)90427-7. View

10.

Huang A . Defective interfering viruses. Annu Rev Microbiol. 1973; 27:101-17. DOI: 10.1146/annurev.mi.27.100173.000533. View

11.

Leamnson R, REICHMANN M . The RNA of defective vesicular stomatitis virus particles in relation to viral cistrons. J Mol Biol. 1974; 85(4):551-68. DOI: 10.1016/0022-2836(74)90315-5. View

12.

Stamminger G, Lazzarini R . Analysis of the RNA of defective VSV particles. Cell. 1974; 3(1):85-93. DOI: 10.1016/0092-8674(74)90044-0. View

13.

Derman E, Darnell J . Relationship of chain transcription to poly(A) addition and processing of hnRNA in HeLa cells. Cell. 1974; 3(3):255-64. DOI: 10.1016/0092-8674(74)90140-8. View

14.

Lazzarini R, Weber G, Johnson L, Stamminger G . Covalently linked message and anti-message (genomic) RNA from a defective vesicular stomatitis virus particle. J Mol Biol. 1975; 97(3):289-307. DOI: 10.1016/s0022-2836(75)80042-8. View

15.

Schnitzlein W, REICHMANN M . The size and the cistronic origin of defective vesicular stomatitis virus particle RNAs in relation to homotypic and heterotypic interference. J Mol Biol. 1976; 101(3):307-25. DOI: 10.1016/0022-2836(76)90150-9. View

16.

Colonno R, Banerjee A . A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro. Cell. 1976; 8(2):197-204. DOI: 10.1016/0092-8674(76)90003-9. View

17.

Colonno R, Lazzarini R, Keene J, Banerjee A . In vitro synthesis of messenger RNA by a defective interfering particle of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1977; 74(5):1884-8. PMC: 431036. DOI: 10.1073/pnas.74.5.1884. View

18.

Marcus P, Sekellick M, Johnson L, Lazzarini R . Cell killing by viruses. V. Transcribing defective interfering particles of vesicular stomatitis virus function as cell-killing particles. Virology. 1977; 82(1):242-6. DOI: 10.1016/0042-6822(77)90048-4. View

19.

Donis-Keller H, Maxam A, Gilbert W . Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977; 4(8):2527-38. PMC: 342589. DOI: 10.1093/nar/4.8.2527. View

20.

Leppert M, Kort L, Kolakofsky D . Further characterization of Sendai virus DI-RNAs: a model for their generation. Cell. 1977; 12(2):539-52. DOI: 10.1016/0092-8674(77)90130-1. View