A Versatile Primer for DNA Sequencing in the M13mp2 Cloning System

Overview

Journal Gene

Specialty Molecular Biology

Date 1980 Jun 1

PMID 6250947

Citations 95

Authors

G Heidecker

J Messing

B Gronenborn

Affiliations

Soon will be listed here.

Abstract

A primer for DNA sequencing by the chain-termination method in the M13mp2 cloning system was constructed and amplified. The primer was isolated as an EcoRI/AluI restriction fragment. After conversion of the AluI end into an EcoRI end the fragment was cloned in pBR325 from which it can be recovered by cleavage with EcoRI. The primer hybridizes to the single-stranded DNA of the mature M13mp2 phage next to the site of insertion thereby directing DNA synthesis along the inserted DNA.

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