Isolation of the Chicken Thymidine Kinase Gene by Plasmid Rescue

Overview

Journal Nature

Specialty Science

Date 1980 May 22

PMID 6246445

Citations 80

Authors

M Perucho

D Hanahan

L Lipsich

M Wigler

Affiliations

Soon will be listed here.

Abstract

We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in BR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.

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