Cell-substratum Attachment and Cell Surface Hyaluronate of Rous Sarcoma Virus-transformed Chondrocytes

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1980 May 1

PMID 6246121

Citations 2

Authors

Y Mikuni-Takagaki

B P Toole

Affiliations

Soon will be listed here.

Abstract

Hyaluronate is associated with the cell surface of cultured Rous sarcoma virus-transformed chondrocytes. Detachment of these cells from their substratum by a variety of reagents is accompanied by release of 75-100% of this hyaluronate into solution. Treatment of the cells with 200 U/ml protease-free Streptomyces hyaluronidase at 37 degrees C cause release of greater than 90% of the cell surface hyaluronate and complete cell detachment. Treatment with a lower concentration of Streptomyces hyaluronidase (30 U/ml) at 25 degrees C or a corresponding activity of testicular hyaluronidase gives similar results, but only in the presence of mM EGTA. Treatment with the lower activities of either hyaluronidase or with 1 mM EGTA alone release only approximately 45% of the cell surface hyaluronate and does not cause significant cell detachment. It is concluded that there are two populations of cell surface hyaluronate differing in their accessibility or their resistance to dissociation from other components of the cell surface. It is proposed that the less readily released fraction is located between the transformed chondrocyte surface and substratum and is necessary for their interaction.

Citing Articles

Proteoglycans and cell adhesion. Their putative role during tumorigenesis.

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PMID: 6083829 DOI: 10.1007/BF00051458.

Limited cleavage of cellular fibronectin by plasminogen activator purified from transformed cells.

Quigley J, Gold L, SCHWIMMER R, Sullivan L Proc Natl Acad Sci U S A. 1987; 84(9):2776-80.

PMID: 3033662 PMC: 304741. DOI: 10.1073/pnas.84.9.2776.

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