A Simple and Fast Method for the Isolation of Basolateral Plasma Membranes from Rat Small-intestinal Epithelial Cells

Overview

Journal Biochem J

Specialty Biochemistry

Date 1980 Jan 15

PMID 6245639

Citations 44

Authors

V Scalera

C Storelli

W Haase

H Murer

Affiliations

Soon will be listed here.

Abstract

A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na+ + K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.

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