A Spite Specific Endonuclease from Thermus Thermophilus 111, Tth111I

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Jan 11

PMID 6243779

Citations 12

Authors

T Shinomiya

S Sato

Affiliations

Soon will be listed here.

Abstract

A site specific endonuclease with novel specificity has been isolated from Thermus thermophilus strain 111 and named Tth111I. Tth111I cleaves lambda DNA into three fragments of 23.5, 25.7 and 50.8% of the total length, and ColE1 DNA into two fragments of nearly equal length. The sequences around Tth111I cleavage sites of ColE1 and lambda DNA were determined by the Maxam and Gilbert method and the two dimensional mapping method. The results suggest that Tth111I recognizes the DNA sequence (formula: see text) and cleaves the site as indicated by the arrows. Assuming that the first T.A pair in the sequence can be replaced for any base pair, the Tth111I recognition sequence has the symmetry with the two-fold axis as most type II restriction endonucleases do.

Citing Articles

Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.

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Two different isoschizomers of the type-II restriction endonuclease Taq I (T/CGA) within the same Thermus isolate: Tsp32 I, an enzyme with similar heat stability properties to the prototype enzyme Taq I, and Tsp32 II, a hyperthermostable....

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Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1.

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A sequence-specific endonuclease (Xmn I) from Xanthomonas manihotis.

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A second site specific endonuclease from Thermus thermophilus 111, Tth111II.

Shinomiya T, Kobayashi M, Sato S Nucleic Acids Res. 1980; 8(15):3275-85.

PMID: 6255411 PMC: 324152. DOI: 10.1093/nar/8.15.3275.

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