Comparison of the High-mobility-group Chromosomal Proteins in Rainbow-trout (Salmo Gairdnerii) Liver and Testis

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Dec 1

PMID 6229248

Citations 3

Authors

E Brown

G H Goodwin

Affiliations

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Abstract

Chromatography and characterization of the proteins extracted by 5% (w/v) HClO4 from rainbow-trout (Salmo gairdnerii) liver and testis show that the two tissues present a characteristically different spectrum of high-mobility-group (HMG) proteins. A variant subfraction of HMG C is found in liver, but is not detectable in testis, where even the main fraction of HMG C is present in only very low quantity. A protein, F, which appears to be related to protein H6 has similarly been isolated only from liver and not from testis. Quantification of the HMG proteins in total 5%-HClO4 extracts of trout liver and testis nuclei shows that, in relation to DNA, levels of HMG T1 and T2, and D are more than 2-fold, and C, 20-fold higher in liver than in testis. However, these differences do not result merely from the sequential withdrawal of HMG proteins at the same time that histones are replaced by protamines in the developing spermatid, since in testis, at some stages of maturation, levels of H6 are almost 2-fold higher than in liver. The implications of these findings for the function of HMG proteins are discussed.

Citing Articles

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References

Kuehl L, DIXON G . The release of high mobility group protein H6 and protamine gene sequences upon selective DNase I degradation of trout testis chromatin. Nucleic Acids Res. 1980; 8(13):2859-69. PMC: 324130. DOI: 10.1093/nar/8.13.2859. View

Mathew C, Goodwin G, JOHNS E . The protein composition of rat satellite chromatin. FEBS Lett. 1981; 125(1):25-9. DOI: 10.1016/0014-5793(81)80988-x. View

Brown E, Goodwin G, Mayes E, HASTINGS J, JOHNS E . Heterogeneity of proteins resembling high-mobility-group protein HMG-T in trout testes nuclei. Biochem J. 1980; 191(2):661-4. PMC: 1162261. DOI: 10.1042/bj1910661. View

Christensen M, DIXON G . Comparison of the high mobility group proteins and their chromatin distribution in trout testis and liver. J Biol Chem. 1981; 256(14):7549-56. View

Bhullar B, Hewitt J, Candido E . The large high mobility group proteins of rainbow trout are localized predominantly in the nucleus and nucleoli of a cultured trout cell line. J Biol Chem. 1981; 256(16):8801-6. View

Levinger L, Barsoum J, Varshavsky A . Two-dimensional hybridization mapping of nucleosomes. comparison of DNA and protein patterns. J Mol Biol. 1981; 146(3):287-304. DOI: 10.1016/0022-2836(81)90389-2. View

Goodwin G, Wright C, JOHNS E . The characterisation of 1SF monomer nucleosomes from hen oviduct and the partial characterisation of a third HMG14/17-like in such nucleosomes. Nucleic Acids Res. 1981; 9(12):2761-75. PMC: 326891. DOI: 10.1093/nar/9.12.2761. View

Gordon J, Kaufman R, Rosenfeld B . Independent control during myogenesis of histone and high-mobility-group (HMGs) chromosomal protein heterogeneity. Arch Biochem Biophys. 1981; 211(2):709-21. DOI: 10.1016/0003-9861(81)90507-5. View

Lund T, Holtlund J, Kristensen T, ostvold A, Sletten K, LALAND S . HMG 17 in metaphase-arrested and interphase HeLa S3 cells. FEBS Lett. 1981; 133(1):84-8. DOI: 10.1016/0014-5793(81)80476-0. View

10.

Reudelhuber T, Ball D, Davis A, Garrard W . Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA. Nucleic Acids Res. 1982; 10(4):1311-25. PMC: 320527. DOI: 10.1093/nar/10.4.1311. View

11.

Brown E, Goodwin G . Isolation from trout liver of a methionine-containing H1 subfraction, THImet. Eur J Biochem. 1983; 134(2):255-9. DOI: 10.1111/j.1432-1033.1983.tb07559.x. View

12.

Ceriotti G . A microchemical determination of desoxyribonucleic acid. J Biol Chem. 1952; 198(1):297-303. View

13.

Wigle D, DIXON G . A new histone from trout testis. J Biol Chem. 1971; 246(18):5636-44. View

14.

Goodwin G, Sanders C, JOHNS E . A new group of chromatin-associated proteins with a high content of acidic and basic amino acids. Eur J Biochem. 1973; 38(1):14-9. DOI: 10.1111/j.1432-1033.1973.tb03026.x. View

15.

Levy B, DIXON G . Diversity of sequences of polyadenylated cytoplasmic RNA from rainbow trout (Salmo gairdnerii) testis and liver. Biochemistry. 1977; 16(5):958-64. DOI: 10.1021/bi00624a023. View

16.

Levy W B, Wong N, DIXON G . Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes. Proc Natl Acad Sci U S A. 1977; 74(7):2810-4. PMC: 431301. DOI: 10.1073/pnas.74.7.2810. View

17.

Rabbani A, Goodwin G, JOHNS E . High mobility group non-histone chromosomal proteins from chicken erythrocytes. Biochem Biophys Res Commun. 1978; 81(2):351-8. DOI: 10.1016/0006-291x(78)91540-1. View

18.

Smith B, Robertson D, BIRBECK M, Goodwin G, JOHNS E . Immunochemical studies of high mobility group non-histone chromatin proteins HMG 1 and HMG 2. Exp Cell Res. 1978; 115(2):420-3. DOI: 10.1016/0014-4827(78)90298-7. View

19.

Weisbrod S, Weintraub H . Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin. Proc Natl Acad Sci U S A. 1979; 76(2):630-4. PMC: 383002. DOI: 10.1073/pnas.76.2.630. View

20.

Mathew C, Goodwin G, Gooderham K, Walker J, JOHNS E . A comparison of the high mobility group non-histone chromatin protein HMG 2 in chicken thymus and erythrocytes. Biochem Biophys Res Commun. 1979; 87(4):1243-51. DOI: 10.1016/s0006-291x(79)80040-6. View