Correlation of Lysosomal Enzyme Abnormalities in Various Forms of Adult Leukaemia

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1983 Sep 1

PMID 6224822

Citations 6

Authors

G T Besley

S E Moss

A D Bain

A E Dewar

Affiliations

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Abstract

Lysosomal enzyme activities were studied in cells derived from the following types of leukaemia: chronic myeloid, acute myeloid, acute myelomonocytic, acute monocytic, non-T, non-B cell acute lymphoblastic, T-cell acute lymphoblastic, B-cell chronic lymphocytic and T-cell chronic lymphocytic. Activities of beta-hexosaminidase and alpha-mannosidase were significantly higher in cells from acute monocytic and acute myelomonocytic leukaemias, and somewhat higher in the other myeloid leukaemias, when compared with control granulocytes. Activities of beta-hexosaminidase, alpha-mannosidase, alpha-fucosidase, beta-glucuronidase and acid phosphatase were markedly lower in B cells of chronic lymphocytic leukaemia when compared with control or other leukaemic lymphoid cells. On isoelectric focusing abnormal patterns of beta-hexosaminidase, alpha-mannosidase and beta-glucuronidase activities were commonly found in myeloid and non-T, non-B cell leukaemias. All patients with acute myeloid leukaemia exhibited a relative decrease in the B form of beta-hexosaminidase activity. The results described show that studies on lysosomal enzymes may assist in the classification of different types of leukaemia.

Citing Articles

Effect of all trans retinoic acid on lysosomal alpha-D-mannosidase activity in HL-60 cell: correlation with HL-60 cells differentiation.

Racanicchi L, Montanucci P, Basta G, Pensato A, Conti V, Calafiore R Mol Cell Biochem. 2007; 308(1-2):17-24.

PMID: 17899319 DOI: 10.1007/s11010-007-9606-3.

Device to collect fibrils of collagen from biopsies of human skin.

GELOS U J Clin Pathol. 1985; 38(9):1077-8.

PMID: 4044875 PMC: 499367. DOI: 10.1136/jcp.38.9.1077.

Enzyme markers in acute non-lymphoid leukaemia.

Besley G, Moss S, Dewar A, Eden O J Clin Pathol. 1985; 38(9):1076-7.

PMID: 3862679 PMC: 499366. DOI: 10.1136/jcp.38.9.1076-c.

Lysosomal enzyme abnormalities in preleukaemic Sweet's disease: case report.

Judge M, Moss S, Besley G, Parker A J Clin Pathol. 1987; 40(1):67-9.

PMID: 3469219 PMC: 1140830. DOI: 10.1136/jcp.40.1.67.

Ultrastructural studies of a lysosomal enzyme during lymphocyte activation.

Bou-Gharios G, Moss J, Abraham D, Partridge T, Olsen I Br J Exp Pathol. 1988; 69(5):661-70.

PMID: 3264180 PMC: 2013276.

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