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Genetic Transformation of Putative Cariogenic Properties in Streptococcus Mutans

Overview
Journal Infect Immun
Publisher American Society for Microbiology
Specialty Allergy & Immunology
Date 1983 Aug 1
PMID 6223884
Citations 49
Authors
D Perry
L M Wondrack
H K Kuramitsu
Affiliations
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Abstract

Rough colonial morphology and bacteriocin production, two properties which may be associated with the cariogenicity of Streptococcus mutans, were transformed into several strain GS-5 mutants defective in each respective property. Transformation was determined by observing the frequency of cotransfer of these properties with different reference markers. The rough colonial transformants were identical to the parental GS-5 strain with respect to ability to synthesize water-insoluble glucans and undergo in vitro sucrose-dependent colonization of glass surfaces. Alterations in the growth medium and the concentration of the initial cell inoculum resulted in an approximate 10-fold increase in the frequency of transformation of strain GS-5 compared to previous observations.

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References
1.
Freedman M, Tanzer J . Dissociation of plaque formation from glucan-induced agglutination in mutants of Streptococcus mutans. Infect Immun. 1974; 10(1):189-96. PMC: 414976. DOI: 10.1128/iai.10.1.189-196.1974. View
2.
Terleckyj B, SHOCKMAN G . Amino acid requirements of Streptococcus mutans and other oral streptococci. Infect Immun. 1975; 11(4):656-64. PMC: 415118. DOI: 10.1128/iai.11.4.656-664.1975. View
3.
Gibbons R, van Houte J . Dental caries. Annu Rev Med. 1975; 26:121-36. DOI: 10.1146/annurev.me.26.020175.001005. View
4.
Kuramitsu H . Characterization of extracellular glucosyltransferase activity of Steptococcus mutans. Infect Immun. 1975; 12(4):738-49. PMC: 415350. DOI: 10.1128/iai.12.4.738-749.1975. View
5.
Johnson M, Bozzola J, SHECHMEISTER I, SHKLAIR I . Biochemical study of the relationship of extracellular glucan to adherence and cariogenicity in Streptococcus mutans and an extracellular polysaccharide mutant. J Bacteriol. 1977; 129(1):351-7. PMC: 234933. DOI: 10.1128/jb.129.1.351-357.1977. View