Bacteriophage Lambda Receptor Protein in Escherichia Coli K-12: Lowered Affinity of Some Mutant Proteins for Maltose-binding Protein in Vitro

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Feb 1

PMID 6218155

Citations 4

Authors

M Luckey

H Nikaido

Affiliations

Soon will be listed here.

Abstract

Mutant and wild-type LamB proteins (phage lambda receptor proteins) were purified by affinity chromatography with immobilized maltose-binding protein, and their transport functions were tested in reconstituted liposomes. Two mutant proteins exhibited a marked decrease in affinity for immobilized maltose-binding protein, as well as altered transport rates.

Citing Articles

Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation.

Boos W, Shuman H Microbiol Mol Biol Rev. 1998; 62(1):204-29.

PMID: 9529892 PMC: 98911. DOI: 10.1128/MMBR.62.1.204-229.1998.

A mutant form of maltose-binding protein of Escherichia coli deficient in its interaction with the bacteriophage lambda receptor protein.

Bavoil P, Wandersman C, Schwartz M, Nikaido H J Bacteriol. 1983; 155(2):919-21.

PMID: 6223921 PMC: 217771. DOI: 10.1128/jb.155.2.919-921.1983.

Monoclonal antibodies reveal lamB antigenic determinants on both faces of the Escherichia coli outer membrane.

Schenkman S, COUTURE E, Schwartz M J Bacteriol. 1983; 155(3):1382-92.

PMID: 6193098 PMC: 217838. DOI: 10.1128/jb.155.3.1382-1392.1983.

Molecular basis of bacterial outer membrane permeability.

Nikaido H, Vaara M Microbiol Rev. 1985; 49(1):1-32.

PMID: 2580220 PMC: 373015. DOI: 10.1128/mr.49.1.1-32.1985.

References

Sandermann Jr H, Strominger J . Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus. J Biol Chem. 1972; 247(16):5123-31. View

Szmelcman S, Hofnung M . Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor. J Bacteriol. 1975; 124(1):112-8. PMC: 235871. DOI: 10.1128/jb.124.1.112-118.1975. View

Smit J, Kamio Y, Nikaido H . Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants. J Bacteriol. 1975; 124(2):942-58. PMC: 235985. DOI: 10.1128/jb.124.2.942-958.1975. View

Lugtenberg B, Meijers J, Peters R, van der Hoek P, van Alphen L . Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975; 58(1):254-8. DOI: 10.1016/0014-5793(75)80272-9. View

Hofnung M, Jezierska A . lamB mutations in E. coli K12: growth of lambda host range mutants and effect of nonsense suppressors. Mol Gen Genet. 1976; 145(2):207-13. DOI: 10.1007/BF00269595. View

von Meyenburg K, Nikaido H . Outer membrane of gram-negative bacteria. XVII. Secificity of transport process catalyzed by the lambda-receptor protein in Escherichia coli. Biochem Biophys Res Commun. 1977; 78(3):1100-7. DOI: 10.1016/0006-291x(77)90534-4. View

Nakae T . A porin activity of purified lambda-receptor protein from Escherichia coli in reconstituted vesicle membranes. Biochem Biophys Res Commun. 1979; 88(3):774-81. DOI: 10.1016/0006-291x(79)91475-x. View

Wandersman C, Schwartz M, Ferenci T . Escherichia coli mutants impaired in maltodextrin transport. J Bacteriol. 1979; 140(1):1-13. PMC: 216772. DOI: 10.1128/jb.140.1.1-13.1979. View

Luckey M, Nikaido H . Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli. Proc Natl Acad Sci U S A. 1980; 77(1):167-71. PMC: 348229. DOI: 10.1073/pnas.77.1.167. View

10.

Heuzenroeder M, Reeves P . Periplasmic maltose-binding protein confers specificity on the outer membrane maltose pore of Escherichia coli. J Bacteriol. 1980; 141(2):431-5. PMC: 293644. DOI: 10.1128/jb.141.2.431-435.1980. View

11.

Luckey M, Nikaido H . Diffusion of solutes through channels produced by phage lambda receptor protein of Escherichia coli: inhibition by higher oligosaccharides of maltose series. Biochem Biophys Res Commun. 1980; 93(1):166-71. DOI: 10.1016/s0006-291x(80)80261-0. View

12.

Ferenci T, Boos W . The role of the Escherichia coli lambda receptor in the transport of maltose and maltodextrins. J Supramol Struct. 1980; 13(1):101-16. DOI: 10.1002/jss.400130110. View

13.

Nikaido H, Luckey M, Rosenberg E . Nonspecific and specific diffusion channels in the outer membrane of Escherichia coli. J Supramol Struct. 1980; 13(3):305-13. DOI: 10.1002/jss.400130304. View

14.

Nikaido H, Rosenberg E . Effect on solute size on diffusion rates through the transmembrane pores of the outer membrane of Escherichia coli. J Gen Physiol. 1981; 77(2):121-35. PMC: 2215425. DOI: 10.1085/jgp.77.2.121. View

15.

Bavoil P, Nikaido H . Physical interaction between the phage lambda receptor protein and the carrier-immobilized maltose-binding protein of Escherichia coli. J Biol Chem. 1981; 256(22):11385-8. View

16.

Wandersman C, Schwartz M . Mutations that alter the transport function of the LamB protein in Escherichia coli. J Bacteriol. 1982; 151(1):15-21. PMC: 220177. DOI: 10.1128/jb.151.1.15-21.1982. View

17.

CLEMENT J, Hofnung M . Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12. Cell. 1981; 27(3 Pt 2):507-14. DOI: 10.1016/0092-8674(81)90392-5. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View