Effect of Bacterial Host Repair Systems on the Viability of Hydroxylamine and Methyl Methanesulfonate Treated T4 and Lambda Bacteriophages

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1982 Jan 1

PMID 6214698

Citations 3

Authors

C Janion

Affiliations

Soon will be listed here.

Abstract

Survival of HA1 or MMS-treated T4 and lambda phages was estimated in bacterial cells differing in their ability to repair DNA. It has been found that the mismatch repair system of the bacterial host, which involves mutS mutR MutL uvrE and dam loci, does not excise, or does so to only a limited extent, the nonpaired bases from DNA of HA or MMS-treated phages. Mutation in polA, both in the polymerase as well as in the 5' leads to 3' exonuclease activity, have a small effect on survival of HA-treated phages, whereas mutation in the polymerase activity has a pronounced effect on survival of MMS-treated phages. There was a difference in the effect of polA mutations on survival of MMS-treated T4 and lambda phages; the survival of the former was less affected than the latter. Induction of SOS response has no effect on repair of HA and MMS-treated phages. Pretreatment of bacterial host (including the ada- mutant) with low doses of alkylating agents increases the survival of MMS (but not HA)-treated phages; pretreatment of bacteria with HA has no effect on survival of HA-treated phages. Three lines of evidence: the different inactivation rates of MMS-treated T4 and lambda phages, variation in the effect of polA mutations on survival of T4 and lambda phages, and a different level of adaptive response in ada- cells towards of MMS-treated T4 and lambda phages, suggest that the patterns of DNA methylation in T4 and lambda phages are different.

Citing Articles

Rates of spontaneous mutation in bacteriophage T4 are independent of host fidelity determinants.

SANTOS M, Drake J Genetics. 1994; 138(3):553-64.

PMID: 7851754 PMC: 1206207. DOI: 10.1093/genetics/138.3.553.

The frequency of MMS-induced, umuDC-dependent, mutations declines during starvation in Escherichia coli.

Grzesiuk E, Janion C Mol Gen Genet. 1994; 245(4):486-92.

PMID: 7808398 DOI: 10.1007/BF00302261.

Inducible reactivation of bacteriophage T7 damaged by methyl methanesulfonate or UV light.

Dodson L, Masker W J Bacteriol. 1983; 156(1):13-8.

PMID: 6352671 PMC: 215045. DOI: 10.1128/jb.156.1.13-18.1983.

References

Radany E, Friedberg E . A pyrimidine dimer-DNA glycosylase activity associated with the v gene product of bacterophage T4. Nature. 1980; 286(5769):182-5. DOI: 10.1038/286182a0. View

Kenyon C, Walker G . Expression of the E. coli uvrA gene is inducible. Nature. 1981; 289(5800):808-10. DOI: 10.1038/289808a0. View

Margison G, Pegg A . Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts. Proc Natl Acad Sci U S A. 1981; 78(2):861-5. PMC: 319903. DOI: 10.1073/pnas.78.2.861. View

Montesano R, Bresil H, Margison G, Pegg A . Effect of chronic treatment of rats with dimethylnitrosamine on the removal of O6-methylguanine from DNA. Cancer Res. 1980; 40(2):452-8. View

WITKIN E . Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976; 40(4):869-907. PMC: 413988. DOI: 10.1128/br.40.4.869-907.1976. View

Janion C, SHUGAR D . Mutagenicity of hydroxylamine: reaction with analogues of cytosine, 5(6)-substituted cytosines and some 2-keto-4-ethoxypyrimidines. Acta Biochim Pol. 1965; 12(4):337-55. View

Laval J, Pierre J, Laval F . Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli. Proc Natl Acad Sci U S A. 1981; 78(2):852-5. PMC: 319901. DOI: 10.1073/pnas.78.2.852. View

Smirnov G, Favorskaya Y, SKAVRONSKAYA A . Monofunctional alkylating agent-induced inactivation, mutagenesis and DNA degradation in an Escherichia coli mutant deficient in DNA polymerase. Mol Gen Genet. 1971; 111(4):357-67. DOI: 10.1007/BF00569788. View

Cairns J, Robins P, Sedgwick B, Talmud P . The inducible repair of alkylated DNA. Prog Nucleic Acid Res Mol Biol. 1981; 26:237-44. DOI: 10.1016/s0079-6603(08)60408-0. View

10.

Kondo S . Indirect mutagenesis in phage lambda by ultraviolet preirradiation of host bacteria. J Mol Biol. 1975; 97(1):77-92. DOI: 10.1016/s0022-2836(75)80023-4. View

11.

BUDOWSKY E, Sverdlov E, Monastyrskaya G . Mechanism of the mutagenic action of hydroxylamine. IV. Reaction of hydroxylamine and O-methylhydroxylamine with the adenine nucleus. Biochim Biophys Acta. 1971; 246(2):320-8. View

12.

Ishiwata K, Oikawa A . Actions of human DNA glycosylases on uracil-containing DNA, methylated DNA and their reconstituted chromatins. Biochim Biophys Acta. 1979; 563(2):375-84. DOI: 10.1016/0005-2787(79)90056-x. View

13.

Grossman L, Braun A, Feldberg R, MAHLER I . Enzymatic repair of DNA. Annu Rev Biochem. 1975; 44:19-43. DOI: 10.1146/annurev.bi.44.070175.000315. View

14.

Chetsanga C, Lindahl T . Release of 7-methylguanine residues whose imidazole rings have been opened from damaged DNA by a DNA glycosylase from Escherichia coli. Nucleic Acids Res. 1979; 6(11):3673-84. PMC: 327965. DOI: 10.1093/nar/6.11.3673. View

15.

Singer B, Brent T . Human lymphoblasts contain DNA glycosylase activity excising N-3 and N-7 methyl and ethyl purines but not O6-alkylguanines or 1-alkyladenines. Proc Natl Acad Sci U S A. 1981; 78(2):856-60. PMC: 319902. DOI: 10.1073/pnas.78.2.856. View

16.

Singer B . All oxygens in nucleic acids react with carcinogenic ethylating agents. Nature. 1976; 264(5584):333-9. DOI: 10.1038/264333a0. View

17.

Breimer L, Lindahl T . A DNA glycosylase from Escherichia coli that releases free urea from a polydeoxyribonucleotide containing fragments of base residues. Nucleic Acids Res. 1980; 8(24):6199-211. PMC: 328082. DOI: 10.1093/nar/8.24.6199. View

18.

Vogel H, Bonner D . Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956; 218(1):97-106. View

19.

BUDOWSKY E . The mechanism of the mutagenic action of hydroxylamines. Prog Nucleic Acid Res Mol Biol. 1976; 16:125-88. DOI: 10.1016/s0079-6603(08)60757-6. View

20.

Karran P, Lindahl T, Ofsteng I, Evensen G, Seeberg E . Escherichia coli mutants deficient in 3-methyladenine-DNA glycosylase. J Mol Biol. 1980; 140(1):101-27. DOI: 10.1016/0022-2836(80)90358-7. View