Promoter Mapping and Selection of Operator Mutants by Using Insertion of Bacteriophage Mu in the ArgECBH Divergent Operon of Escherichia Coli K-12

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Jul 1

PMID 6211434

Citations 11

Authors

G Beny

A Boyen

D Charlier

W Lissens

A Feller

N Glansdorff

Affiliations

Soon will be listed here.

Abstract

The analysis of a large number of Arg mutants obtained by inserting phage Mu in the argECBH cluster of genes confirmed the "facing" arrangement proposed earlier for the promoters of argE (argEp) and argCBH (argCBHp) and clarified remaining ambiguities regarding the localization of argEp. Casadaban and Cohen's Mu d lac phages (M. Casadaban and S. N. Cohen, Proc. Natl. Acad. Sci. U.S.A. 76:4530-4533, 1979) were used to construct strains where either an intact or a truncated lacZ gene was fused to argC or argB. Several operator-constitutive mutations could be selected for in such strains; the mutations affected both arms of the cluster, thereby defining one common operator region for both directions of transcription.

Citing Articles

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

PMID: 9729611 PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998.

The regulatory region of the divergent argECBH operon in Escherichia coli K-12.

Piette J, Cunin R, Boyen A, Charlier D, Crabeel M, Van Vliet F Nucleic Acids Res. 1982; 10(24):8031-48.

PMID: 6761650 PMC: 327068. DOI: 10.1093/nar/10.24.8031.

Conditionally transposition-defective derivative of Mu d1(Amp Lac).

Hughes K, Roth J J Bacteriol. 1984; 159(1):130-7.

PMID: 6330026 PMC: 215603. DOI: 10.1128/jb.159.1.130-137.1984.

In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.

Casadaban M, Chou J Proc Natl Acad Sci U S A. 1984; 81(2):535-9.

PMID: 6320194 PMC: 344713. DOI: 10.1073/pnas.81.2.535.

Transcription of Regions within the divergent argECBH operon of Escherichia coli: evidence for lack of an attenuation mechanism.

Beny G, Cunin R, Glansdorff N, Boyen A, Charlier J, KELKER N J Bacteriol. 1982; 151(1):58-61.

PMID: 6211433 PMC: 220190. DOI: 10.1128/jb.151.1.58-61.1982.

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