Plasma Membrane Glycoproteins of Cultured Rheumatoid Synovial Fibroblasts

Overview

Journal Clin Rheumatol

Publisher Springer

Specialty Rheumatology

Date 1983 Jun 1

PMID 6205814

Citations 3

Authors

T Kouri

E Vuorio

Affiliations

Soon will be listed here.

Abstract

Fibroblast cultures were started from synovial tissue samples of 12 rheumatoid arthritic, 9 non-rheumatoid synovitic and 6 control patients. External galactose units of plasma membrane glycoproteins of confluent cells were labelled using the galactose oxidase-tritiated borohydride method. These surface-labelled cells were analyzed for possible differences in their glycoproteins by electrophoresis in SDS-containing polyacrylamide gradient gels. Total cell lysates were separated into 50-60 polypeptide bands. Fluorography of the gels revealed about 20 labelled plasma membrane glycoproteins. Some strain-specific differences were detected between the samples in all the groups, but no correlation with rheumatoid arthritis could be demonstrated.

Citing Articles

Two-dimensional cryostat section electrophoresis: a novel method and its application to the diseased synovial membrane.

Fritz P, Mischlinski A, Wicherek C, Saal J Histochem J. 1994; 26(10):804-16.

PMID: 7883590

Etiology of rheumatoid arthritis.

Kouri T Experientia. 1985; 41(4):434-41.

PMID: 2985424 DOI: 10.1007/BF01966141.

Two-dimensional electrophoresis of synovial tissue, synovial fluid and serum in patients with rheumatoid arthritis and related diseases.

Fritz P, Arold N, Mischlinski A, WISSER H, Oeffinger B, Neuhoff V Rheumatol Int. 1990; 10(5):177-83.

PMID: 2075369 DOI: 10.1007/BF02274830.

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