Antigenic Relationships Among Proteins of Bovine Coronavirus, Human Respiratory Coronavirus OC43, and Mouse Hepatitis Coronavirus A59

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1984 Aug 1

PMID 6205167

Citations 47

Authors

B G Hogue

B King

D A Brian

Affiliations

Soon will be listed here.

Abstract

Antisera prepared against each of three single and one pair of major structural proteins of the bovine coronavirus (Mebus strain) were used in immunoblotting studies to measure cross-reactivity with the structural proteins of the human coronavirus OC43 and the mouse hepatitis coronavirus A59. We conclude that the bovine coronavirus is comprised of four major structural proteins, gp190 (normally present as 120- and 100-kilodalton subunits), gp140, pp52, and gp26. The human coronavirus OC43 has an antigenically homologous counterpart of similar molecular mass to each of these proteins. The mouse hepatitis coronavirus A59 has an antigenically homologous counterpart to only three of these proteins: gp190, pp52 and gp26. There is no counterpart in the mouse virus to the 140-kilodalton glycoprotein, the apparent hemagglutinin of the bovine coronavirus.

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References

Storz J, Rott R . Reactivity of antibodies in human serum with antigens of an enteropathogenic bovine coronavirus. Med Microbiol Immunol. 1981; 169(3):169-78. PMC: 7086857. DOI: 10.1007/BF02123590. View

Lai M, Stohlman S . Comparative analysis of RNA genomes of mouse hepatitis viruses. J Virol. 1981; 38(2):661-70. PMC: 171196. DOI: 10.1128/JVI.38.2.661-670.1981. View

Schmidt O, KENNY G . Polypeptides and functions of antigens from human coronaviruses 229E and OC43. Infect Immun. 1982; 35(2):515-22. PMC: 351070. DOI: 10.1128/iai.35.2.515-522.1982. View

Dennis D, Brian D . RNA-dependent RNA polymerase activity in coronavirus- infected cells. J Virol. 1982; 42(1):153-64. PMC: 256056. DOI: 10.1128/JVI.42.1.153-164.1982. View

King B, Brian D . Bovine coronavirus structural proteins. J Virol. 1982; 42(2):700-7. PMC: 256895. DOI: 10.1128/JVI.42.2.700-707.1982. View

Wege H, Siddell S, Ter Meulen V . The biology and pathogenesis of coronaviruses. Curr Top Microbiol Immunol. 1982; 99:165-200. DOI: 10.1007/978-3-642-68528-6_5. View

Baric R, Stohlman S, Lai M . Characterization of replicative intermediate RNA of mouse hepatitis virus: presence of leader RNA sequences on nascent chains. J Virol. 1983; 48(3):633-40. PMC: 255394. DOI: 10.1128/JVI.48.3.633-640.1983. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Laskey R, Mills A . Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975; 56(2):335-41. DOI: 10.1111/j.1432-1033.1975.tb02238.x. View

10.

Sturman L . I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels. Virology. 1977; 77(2):637-49. PMC: 7131136. DOI: 10.1016/0042-6822(77)90488-3. View

11.

Sturman L, Holmes K . Characterization of coronavirus II. Glycoproteins of the viral envelope: tryptic peptide analysis. Virology. 1977; 77(2):650-60. PMC: 7130763. DOI: 10.1016/0042-6822(77)90489-5. View

12.

Pedersen N, Ward J, Mengeling W . Antigenic relationship of the feline infectious peritonitis virus to coronaviruses of other species. Arch Virol. 1978; 58(1):45-53. PMC: 7087319. DOI: 10.1007/BF01315534. View

13.

Hierholzer J, Broderson J, Murphy F . New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Infect Immun. 1979; 24(2):508-22. PMC: 414330. DOI: 10.1128/iai.24.2.508-522.1979. View

14.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

15.

Stern D, Kennedy S . Coronavirus multiplication strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome. J Virol. 1980; 36(2):440-9. PMC: 353660. DOI: 10.1128/JVI.36.2.440-449.1980. View

16.

Burnette W . "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981; 112(2):195-203. DOI: 10.1016/0003-2697(81)90281-5. View