Serological and Cellular Definition of a New HLA-DR Associated Determinant, MC1, and Its Association with Rheumatoid Arthritis

A new serological HLA-DR associated determinant was defined by a cluster of six B cell alloantisera. This determinant, designated as MC1, was strongly associated with DR1 and DR4 and appeared unrelated to MB, MT, and SB. The antigen frequency of MC1 was 47% in Caucasoids and 43% in Blacks. Mixed leukocyte culture cloning yielded an alloreactive T cell clone with PLT specificity associated with MC1. We have also observed that certain alloreactive clones may also acquire expression of MC1 but not DR4 during long-term culture maintenance in the presence of DR4, MC1-positive feeder cells. Disease association studies showed a significantly increased frequency of MC1 in adult rheumatoid arthritis patients (93.5% vs 47.4% in normals; relative risk 16.1). These findings suggest that MC1 represents a novel HLA-D encoded determinant.