Interaction of Cholecystokinin and Vasoactive Intestinal Polypeptide on Function of Mouse Pancreatic Acini in Vitro

Overview

Journal J Physiol

Specialty Physiology

Date 1984 Apr 1

PMID 6204039

Citations 6

Authors

D B Burnham

D J McChesney

K C Thurston

J A Williams

Affiliations

Soon will be listed here.

Abstract

In isolated mouse pancreatic acini, vasoactive intestinal polypeptide (VIP) and secretin potentiated amylase release stimulated by cholecystokinin (CCK). VIP (1-100 nM) or secretin (100-1000 nM) alone elicited a negligible secretory response, whereas in combination with CCK, these agents induced a significantly larger response. VIP increased maximal amylase release elicited by CCK without affecting the potency with which CCK stimulated secretion. The phosphodiesterase inhibitor, 3-isobutyl-1-methyl xanthine (IBMX), from 0.03-1.0 mM had effects on secretion similar to those of VIP. VIP, IBMX and 8-Br-cyclic AMP, all of which act through or mimic the action of cyclic AMP, potentiated the secretory response to maximal concentrations of CCK, carbamylcholine and the ionophore A23187, all of which act via intracellular calcium. In contrast to amylase release, stimulation of acinar glucose transport by CCK or carbamylcholine was not augmented by VIP, secretin, IBMX or 8-Br-cyclic AMP. The results indicate that for amylase release from mouse pancreas, secretagogues acting via cyclic AMP potentiate those acting via calcium. However, potentiation does not apply to all biological responses of the pancreatic acinus and each response must be studied individually.

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References

Deschodt-Lanckman M, Robberecht P, De Neef P, Labrie F, Christophe J . In vitro interactions of gastrointestinal hormones on cyclic adenosine 3':5'-monophosphate levels and amylase output in the rat pancreas. Gastroenterology. 1975; 68(2):318-25. View

Singh M . Role of cyclic adenosine monophosphate in amylase release from dissociated rat pancreatic acini. J Physiol. 1982; 331:547-55. PMC: 1197767. DOI: 10.1113/jphysiol.1982.sp014390. View

Williams J . Regulation of pancreatic acinar cell function by intracellular calcium. Am J Physiol. 1980; 238(4):G269-79. DOI: 10.1152/ajpgi.1980.238.4.G269. View

Gardner J, Rottman A . Action of cholera toxin on dispersed acini from guinea pig pancreas. Biochim Biophys Acta. 1979; 585(2):250-65. DOI: 10.1016/0304-4165(79)90025-4. View

Peikin S, Rottman A, Batzri S, Gardner J . Kinetics of amylase release by dispersed acini prepared from guinea pig pancreas. Am J Physiol. 1978; 235(6):E743-9. DOI: 10.1152/ajpendo.1978.235.6.E743. View

Jung D . Preparation and application of Procion Yellow starch for amylase assay. Clin Chim Acta. 1980; 100(1):7-11. DOI: 10.1016/0009-8981(80)90179-5. View

Collen M, Sutliff V, Pan G, Gardner J . Postreceptor modulation of action of VIP and secretin on pancreatic enzyme secretion by secretagogues that mobilize cellular calcium. Am J Physiol. 1982; 242(4):G423-8. DOI: 10.1152/ajpgi.1982.242.4.G423. View

Singh M . Calcium and cyclic nucleotide interaction in secretion of amylase from rat pancreas in vitro. J Physiol. 1979; 296:159-76. PMC: 1279070. DOI: 10.1113/jphysiol.1979.sp012997. View

Korman L, Walker M, Gardner J . Action of theophylline on secretagogue-stimulated amylase release from dispersed pancreatic acini. Am J Physiol. 1980; 239(4):G324-G333. DOI: 10.1152/ajpgi.1980.239.4.G324. View

10.

Pan G, Collen M, Gardner J . Action of cholera toxin on dispersed acini from rat pancreas. Post-receptor modulation involving cyclic AMP and calcium. Biochim Biophys Acta. 1982; 720(4):338-45. DOI: 10.1016/0167-4889(82)90110-0. View

11.

Sankaran H, Goldfine I, Bailey A, LICKO V, Williams J . Relationship of cholecystokinin receptor binding to regulation of biological functions in pancreatic acini. Am J Physiol. 1982; 242(3):G250-7. DOI: 10.1152/ajpgi.1982.242.3.G250. View

12.

Williams J, Korc M, Dormer R . Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. Am J Physiol. 1978; 235(5):517-24. DOI: 10.1152/ajpendo.1978.235.5.E517. View

13.

BAUDUIN H, Stock C, Launay J, Vincent D, POTVLIEGE P, GRENIER J . On the secretagogue effect of dibutyryl cyclic AMP in the rat exocrine pancreas. Pflugers Arch. 1977; 372(1):69-76. DOI: 10.1007/BF00582208. View

14.

Schulz I, Stolze H . The exocrine pancreas: the role of secretagogues, cyclic nucleotides, and calcium in enzyme secretion. Annu Rev Physiol. 1980; 42:127-56. DOI: 10.1146/annurev.ph.42.030180.001015. View

15.

Gardner J, Jackson M . Regulation of amylase release from dispersed pancreatic acinar cells. J Physiol. 1977; 270(2):439-54. PMC: 1353522. DOI: 10.1113/jphysiol.1977.sp011961. View

16.

Korc M, Williams J, Goldfine I . Stimulation of the glucose transport system in isolated mouse pancreatic acini by cholecystokinin and analogues. J Biol Chem. 1979; 254(16):7624-9. View

17.

Robberecht P, Deschodt-Lanckman M, Lammens M, De Neff P, Christophe J . "In vitro" effects of secretin and vasoactive intestinal polypeptide on hydrolase secretion and cylic AMP levels in the pancreas of five animal species. A comparison with caerulein. Gastroenterol Clin Biol. 1977; 1(6-7):519-25. View

18.

Bayliss W, Starling E . The mechanism of pancreatic secretion. J Physiol. 1902; 28(5):325-53. PMC: 1540572. DOI: 10.1113/jphysiol.1902.sp000920. View

19.

Heisler S, Grondin G, Forget G . The effect of various secretagogues on accumulation of cyclic AMP and secretion of alpha-amylase from rat exocrine pancreas. Life Sci. 1974; 14(4):631-9. DOI: 10.1016/0024-3205(74)90398-1. View