Detection of Non-A, Non-B Hepatitis Antigen by Immunocytochemical Staining

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 May 1

PMID 6203116

Citations 2

Authors

K H Burk

P E Oefinger

G R Dreesman

Affiliations

Soon will be listed here.

Abstract

Liver tissue obtained from a chimpanzee during the acute phase of an experimental non-A, non-B (NANB) hepatitis virus infection was studied by a sensitive immunocytochemical staining procedure for the presence of NANB viral antigens. Initial investigations were conducted with a model system of hepatitis B virus (HBV) antigens for purposes of comparing two immunocytochemical staining methods. Of these two procedures, an immunoperoxidase procedure, utilizing an avidin-biotinylated enzyme complex, was at least 40-fold more sensitive than a conventional immunoperoxidase technique for the detection of HBV-specific tissue antigens. Utilization of the avidin-biotin-amplified immunoperoxidase staining procedure, in conjunction with four primary convalescent antisera obtained from NANB hepatitis-implicated donors, resulted in the observation of NANB virus-associated antigen in the cytoplasm of hepatocytes from an infected chimpanzee liver. These same human antisera were not reactive with a number of uninfected control cells nor with cells infected with HBV, hepatitis A virus, or cytomegalovirus. Preincubation of one of these convalescent NANB sera, or IgG derived thereof, with an acute-phase serum obtained from a NANB hepatitis virus-infected chimpanzee abolished the antibody reactivity. We conclude from these observations that selected convalescent sera from NANB hepatitis virus-infected patients contain low levels of antibody that specifically react with a cytoplasmic antigen associated with NANB virus-infected hepatocytes.

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References

Bradley D, MAYNARD J, Popper H, Ebert J, Cook E, Fields H . Persistent non-A, non-B hepatitis in experimentally infected chimpanzees. J Infect Dis. 1981; 143(2):210-8. DOI: 10.1093/infdis/143.2.210. View

Suh D, White Y, Eddleston A, Amini S, Tsiquaye K, Zuckerman A . Specificity of an immunoprecipitin test for non-A non-B hepatitis. Lancet. 1981; 1(8213):178-80. DOI: 10.1016/s0140-6736(81)90059-3. View

Chircu L, Pezzella M, Lacava V, Ricci G . Post-transfusion hepatitis: antigen/antibody systems correlated with non-A, non-B hepatitis. J Med Virol. 1980; 6(2):147-51. DOI: 10.1002/jmv.1890060207. View

Burk K, Cabral G, Dreesman G, Peters R, Alter H . Ultrastructural changes and virus-like particles localized in liver hepatocytes of chimpanzees infected with non-A, non-B hepatitis. J Med Virol. 1981; 7(1):1-19. DOI: 10.1002/jmv.1890070102. View

Hsu S, RAINE L, FANGER H . Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-80. DOI: 10.1177/29.4.6166661. View

Oefinger P, Bronson D, Dreesman G . Induction of hepatitis B surface antigen in human hepatoma-derived cell lines. J Gen Virol. 1981; 53(Pt 1):105-13. DOI: 10.1099/0022-1317-53-1-105. View

Hoofnagle J . Precipitin system detected in sera from patients with non-A, non-B hepatitis. J Med Virol. 1981; 7(4):315-9. DOI: 10.1002/jmv.1890070407. View

Mounts P, Shah K, Kashima H . Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx. Proc Natl Acad Sci U S A. 1982; 79(17):5425-9. PMC: 346910. DOI: 10.1073/pnas.79.17.5425. View

Nerurkar L, Jacob A, Madden D, Sever J . Detection of genital herpes simplex infections by a tissue culture-fluorescent-antibody technique with biotin-avidin. J Clin Microbiol. 1983; 17(1):149-54. PMC: 272591. DOI: 10.1128/jcm.17.1.149-154.1983. View

10.

Kendall C, Dreesman G . Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA). J Immunol Methods. 1983; 56(3):329-39. DOI: 10.1016/s0022-1759(83)80022-2. View

11.

Duermeyer W, Stute R, Hellings J . An enzyme-linked immunosorbent assay for an antigen related to non-A, non-B hepatitis and its antibody: partial characterization of the antigen and chimpanzee transmission. J Med Virol. 1983; 11(1):11-21. DOI: 10.1002/jmv.1890110103. View

12.

Prince A . Non-A, non-B hepatitis viruses. Annu Rev Microbiol. 1983; 37:217-32. DOI: 10.1146/annurev.mi.37.100183.001245. View

13.

Alter H, Holland P, Morrow A, Purcell R, Feinstone S, Moritsugu Y . Clinical and serological analysis of transfusion-associated hepatitis. Lancet. 1975; 2(7940):838-41. DOI: 10.1016/s0140-6736(75)90234-2. View

14.

Seeff L, Wright E, ZIMMERMAN H, McCOLLUM R . Va cooperative study of post-transfusion hepatitis, 1969-1974: incidence and characteristics of hepatitis and responsible risk factors. Am J Med Sci. 1975; 270(2):355-62. DOI: 10.1097/00000441-197509000-00018. View

15.

Hoofnagle J, Gerety R, Tabor E, Feinstone S, Barker L, Purcell R . Transmission of non-A, non-B hepatitis. Ann Intern Med. 1977; 87(1):14-20. DOI: 10.7326/0003-4819-87-1-14. View

16.

Hollinger F, Gitnick G, AACH R, SZMUNESS W, Mosley J, Stevens C . Non-A, non-B hepatitis transmission in chimpanzees: a project of the transfusion-transmitted viruses study group. Intervirology. 1978; 10(1):60-8. DOI: 10.1159/000148969. View

17.

Alter H, Purcell R, Holland P, Popper H . Transmissible agent in non-A, non-B hepatitis. Lancet. 1978; 1(8062):459-63. DOI: 10.1016/s0140-6736(78)90131-9. View

18.

Tabor E, Gerety R, Drucker J, Seeff L, Hoofnagle J, Jackson D . Transmission of non-A, non-B hepatitis from man to chimpanzee. Lancet. 1978; 1(8062):463-6. DOI: 10.1016/s0140-6736(78)90132-0. View

19.

Cabral G, Gyorkey F, Gyorkey P, Melnick J, Dreesman G . Immunohistochemical and electron microscopic detection of hepatitis B surface and core antigens. Exp Mol Pathol. 1978; 29(2):156-69. DOI: 10.1016/0014-4800(78)90036-9. View

20.

Shirachi R, Shiraishi H, Tateda A, Kikuchi K, Ishida N . Hepatitis "C" antigen in non-A, non-B post-transfusion hepatitis. Lancet. 1978; 2(8095):853-6. DOI: 10.1016/s0140-6736(78)91567-2. View