Detection of Chlamydia Trachomatis Inclusions in McCoy and HeLa-229 Cells: an Alternative Staining Technique Using Toluidine Blue
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Toluidine blue staining was used to detect Chlamydia trachomatis inclusions in both McCoy and HeLa-229 cells from clinical specimens. This method was more sensitive than iodine staining for detecting C trachomatis inclusions in both McCoy and HeLa-229 cells and also more sensitive than Giemsa staining for detecting chlamydial inclusions in HeLa-229 cells. While its sensitivity for detection of chlamydial inclusions in McCoy cells is equal to that of Giemsa staining, toluidine blue staining is easier and faster to perform.
Recent advances in Chlamydia trachomatis.
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