Murine Monoclonal Antibody to a Single Protein Neutralizes the Infectivity of Human Cytomegalovirus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Feb 1

PMID 6199788

Citations 44

Authors

L E Rasmussen

R M Nelson

D C Kelsall

T C Merigan

Affiliations

Soon will be listed here.

Abstract

Murine monoclonal antibodies to human cytomegalovirus (CMV) strain AD169 were selected that neutralized virus infectivity. One monoclonal antibody-producing hybridoma, 1G6, was used to produce ascites fluid from which immunoglobulin was isolated. This antibody efficiently neutralized CMV AD169, other laboratory strains (Towne, Davis), and clinical isolates of CMV in early tissue culture passage (less than 10) in the absence of complement. The antibody immunoprecipitated a single 86,000-dalton protein from both laboratory and clinical strains. This viral protein was demonstrated by indirect immunofluorescence to be localized in the cytoplasm of CMV-infected cells.

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