7S-K Nuclear RNA from Simian Virus 40-transformed Cells Has Sequence Homology to the Viral Early Promoter

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Dec 1

PMID 6196783

Citations 2

Authors

U Sohn

J SZYSZKO

D Coombs

M Krause

Affiliations

Soon will be listed here.

Abstract

Previous findings in our laboratory have identified a specific small nuclear RNA (7S-K) that promotes transcription initiation by RNA polymerase II in isolated mammalian nuclei. The present study was designed to investigate the homology between 7S-K RNAs and host and viral sequences in simian virus 40 (SV40)-transformed and in untransformed mouse 3T3 cells, with the object of testing the hypothesis that these RNAs take part in the transcription initiation complex by base pairing to promoter/enhancer regions of active genes. DNA . RNA hybridization experiments, using either Southern or RNA blotting techniques, indicated that both 7S-K and 7S-L RNAs hybridize to midrepetitive fractions of the mouse genome. However, only 7S-K RNA from transformed cells hybridized to SV40 DNA. Restriction mapping and nuclease S1 treatment of the hybridized region of SV40 yielded a 45-nucleotide-long hybrid duplex. Partial sequence analysis of the 5' end of the DNA in this duplex revealed sequence homology with the 21-base-pair repeat sequence, identified as the SV40 early promoter. Because the viral early gene is expressed in transformed cells, we conclude that 7S-K RNAs in these cells contain a species that has sequence homology to a promoter of an active gene. Taking these results together with previous ones, we postulate that the observed stimulatory activity of 7S-K RNA on transcription initiation is due to its recognition of promoter sequences, either to facilitate the formation or as part of the transcription initiation complex.

Citing Articles

Localization of small RNAs hybridizable to a B2 clone in the nuclear fraction of mouse cell lines.

Gallant J, Lovely J, Krause M Mol Biol Rep. 1987; 12(1):49-53.

PMID: 2442600 DOI: 10.1007/BF00580650.

Simian virus 40 DNA replication in vitro: study of events preceding elongation of chains.

Wobbe C, Dean F, Murakami Y, Weissbach L, Hurwitz J Proc Natl Acad Sci U S A. 1986; 83(13):4612-6.

PMID: 2425351 PMC: 323791. DOI: 10.1073/pnas.83.13.4612.

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