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Efficient Extraction of RNA from Mammalian Tissue

Overview
Journal Mol Cell Biochem
Publisher Springer
Specialty Biochemistry
Date 1983 Jan 1
PMID 6196612
Citations 14
Authors
M L Frazier
W Mars
D L Florine
R A Montagna
G F Saunders
Affiliations
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Abstract

RNA extraction from mammalian tissue has been compared using the different deproteinizing agents: a) guanidine-HCl, b) guanidinium-thiocyanate, c) buffer-saturated phenol, or d) buffer-saturated phenol followed by a proteinase K digestion of the aqueous phase. Both solid tissues (first, second, and third trimester fetal bovine pancreas), and human white blood cell populations were studied. Degradation, as seen in citric acid-urea agarose gels, and the ability to serve as templates for cell-free protein synthesis were used as criteria to assess the efficiency of the different methods. We conclude that employing buffer-saturated phenol with proteinase K digestion is a superior method for consistent extraction of relatively undegraded RNA in quantitative amounts from mammalian tissue.

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