Purines As 'hyper-repressors' of Glucose Transport. A Role for Phosphoribosyl Diphosphate

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Jul 15

PMID 6193780

Citations 1

Authors

R J Gay

H Amos

Affiliations

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Abstract

Under selected conditions the rate of glucose transport and the intracellular phosphoribosyl diphosphate (PPRibP) concentrations of chick-embryo fibroblasts are inversely correlated. This relationship holds when cells are incubated with mannose, fructose, xylose or various concentrations of glucose. The metabolic inhibitors 2,4-dinitrophenol, rotenone and Methylene Blue increased glucose transport and decreased PPRibP. The addition of any pyrimidine or purine base or ribonucleoside dramatically depleted PPRibP pools, regardless of the carbon source. Addition of guanine (10 microM) or hypoxanthine (100 microM) decreased transport in glucose-grown chick cells to barely detectable values, but did not affect increases observed in cells depressed by substitution of xylose for glucose. Guanosine, inosine and the purine analogues 6-thioguanine, 6-thioguanosine, 8-azaguanine and 6-methylmercaptopurine riboside sharply decreased transport in glucose-grown cells and blocked the increase in transport resulting from the replacement of glucose by fructose or xylose in the culture medium.

Citing Articles

Mutations in the phosphoglucose isomerase gene can lead to marked alterations in cellular ATP levels in cultured fibroblasts exposed to simple nutrient shifts.

PLESNER P, Ullrey D, KALCKAR H Proc Natl Acad Sci U S A. 1985; 82(9):2761-3.

PMID: 3857613 PMC: 397645. DOI: 10.1073/pnas.82.9.2761.

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