Effects of Bleomycin and Methylprednisolone on the Biosynthesis of Oligosaccharide-lipids and Glycoproteins in Lung

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Sep 1

PMID 6193779

Citations 3

Authors

N Mohapatra

W S Lynn

S N Bhattacharyya

Affiliations

Soon will be listed here.

Abstract

Tissues from rabbit lung were found to incorporate radioactivity from [3H]mannose into the oligosaccharide of a polar lipid soluble in chloroform/methanol/water (10:10:3, by vol.). Only one oligosaccharide-lipid was formed and the composition of the radiolabelled carbohydrate moiety was Glc3Man9(GlcNAc)2. An antitumour antibiotic, bleomycin, and an anti-inflammatory steroid, methylprednisolone, partially inhibited the incorporation of [3H]mannose into oligosaccharide-lipids and in addition resulted in the production of two new components, Man5(GlcNAc)2 and Man2(GlcNAc)2. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of the glycoproteins synthesized from the oligosaccharide-lipid intermediates showed labelled components with Mr greater than 200000, 130000, 80000 and 62000. The addition of the drugs resulted in the same pattern with the addition of a new component of Mr 36000. Endoglucosaminidase H-treatment of the glycopeptides isolated from the Pronase-digested glycoproteins indicated that the oligosaccharide units in the glycoproteins were of the high-mannose-type.

Citing Articles

Fibronectin: source of mannose in a highly purified respiratory mucin.

Bhattacharyya S, Kaufman B, Khorrami A, Enriquez J, Manna B Inflammation. 1988; 12(5):433-46.

PMID: 3198249 DOI: 10.1007/BF00919437.

Neutral and acidic human tracheobronchial mucin. Isolation and characterization of core protein.

Bhattacharyya S, Veit B, Manna B, Enriquez J, Walker M, Khorrami A Inflammation. 1990; 14(4):355-73.

PMID: 2379952 DOI: 10.1007/BF00914088.

Deglycosylation of neutral and acidic human colonic mucin.

Bhattacharyya S, Enriquez Sr J, Manna B Inflammation. 1990; 14(1):93-107.

PMID: 2323808 DOI: 10.1007/BF00914033.

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