» Articles » PMID: 6193758

Rapid Isolation of DNA from Staphylococcus Aureus

Overview
Date 1983 Jul 1
PMID 6193758
Citations 57
Authors
Affiliations
Soon will be listed here.
Abstract

We describe a Staphylococcus aureus bulk DNA isolation procedure which uses detergent and guanidine hydrochloride to free the nucleic acid from contaminants. The procedure is rapid and yields high-molecular-weight DNA suitable for molecular biological procedures.

Citing Articles

Phylogenomic Analyses of Three Distinct Lineages Uniting and from Diverse Hosts.

House L, Hasan A, Asnayanti A, Alrubaye A, Pummill J, Rhoads D Microorganisms. 2024; 12(8).

PMID: 39203392 PMC: 11356006. DOI: 10.3390/microorganisms12081549.


Occurrence of -Positive Linezolid-Susceptible and Non- Staphylococcal Isolates from Pig Farms.

Lee G, Yang S Antibiotics (Basel). 2023; 12(2).

PMID: 36830270 PMC: 9952267. DOI: 10.3390/antibiotics12020359.


Role of the Staphylococcus aureus Extracellular Loop of GraS in Resistance to Distinct Human Defense Peptides in PMN and Invasive Cardiovascular infections.

Cheung A, Cho J, Bayer A, Yeaman M, Xiong Y, Donegan N Infect Immun. 2021; 89(10):e0034721.

PMID: 34227840 PMC: 8445198. DOI: 10.1128/IAI.00347-21.


Genetic Factors Associated with Increased Host Defense Antimicrobial Peptide Resistance in Sequence Type 5 Healthcare-Associated MRSA Clinical Isolates.

Kang K, Lee G, Yang S Biomolecules. 2020; 10(10).

PMID: 33036325 PMC: 7599770. DOI: 10.3390/biom10101415.


Whole-Genome Comparisons of Isolates from Cattle and Chickens.

Shwani A, Adkins P, Ekesi N, Alrubaye A, Calcutt M, Middleton J Appl Environ Microbiol. 2020; 86(12).

PMID: 32245765 PMC: 7267197. DOI: 10.1128/AEM.00484-20.


References
1.
Radloff R, BAUER W, Vinograd J . A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967; 57(5):1514-21. PMC: 224502. DOI: 10.1073/pnas.57.5.1514. View

2.
NOVICK R . Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967; 33(1):155-66. DOI: 10.1016/0042-6822(67)90105-5. View

3.
Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

4.
Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

5.
Novick R, Murphy E, Gryczan T, Baron E, Edelman I . Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979; 2(1):109-29. DOI: 10.1016/0147-619x(79)90010-6. View