Prospective Study of Gram-stained Stool Smears in Diagnosis of Clostridium Difficile Colitis
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Gram stains of stools from patients with diarrhea and control patients with no diarrhea were examined for a predominance of gram-positive rods and the presence of polymorphonuclear leukocytes. Results were compared with those from lower gastrointestinal endoscopy for pseudomembranes. Clostridium difficile culturing, and C. difficile toxin assay. The Gram stain was moderately difficult to interpret and was not useful in diagnosing diarrheal disease associated with C. difficile.
Culturing and maintaining Clostridium difficile in an anaerobic environment.
Edwards A, Suarez J, McBride S J Vis Exp. 2013; (79):e50787.
PMID: 24084491 PMC: 3871928. DOI: 10.3791/50787.
Becker S, Vogt J, Knopp S, Panning M, Warhurst D, Polman K BMC Infect Dis. 2013; 13:37.
PMID: 23347408 PMC: 3579720. DOI: 10.1186/1471-2334-13-37.
Is Fecal Leukocyte Test a good predictor of Clostridium difficile associated diarrhea?.
Reddymasu S, Sheth A, Banks D Ann Clin Microbiol Antimicrob. 2006; 5:9.
PMID: 16623943 PMC: 1459191. DOI: 10.1186/1476-0711-5-9.
Toxin gene analysis of a variant strain of Clostridium difficile that causes human clinical disease.
Sambol S, Merrigan M, Lyerly D, Gerding D, Johnson S Infect Immun. 2000; 68(10):5480-7.
PMID: 10992443 PMC: 101495. DOI: 10.1128/IAI.68.10.5480-5487.2000.
Detection of Clostridium difficile in faeces by direct gas liquid chromatography.
Levett P J Clin Pathol. 1984; 37(2):117-9.
PMID: 6693575 PMC: 498663. DOI: 10.1136/jcp.37.2.117.