Transformation of LMTK- Cells with Purified HLA Class I Genes. II. Serologic Characterization of HLA-A3 and CW3 Molecules

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1983 Jan 1

PMID 6190745

Citations 7

Authors

F A Lemonnier

P C Dubreuil

C Layet

M Malissen

D Bourel

P Mercier

B K Jakobsen

D H Caillol

A Svejgaard

F M KOURILSKY

B R Jordan

Affiliations

Soon will be listed here.

Abstract

The expression of two different HLA class I genes was observed after transformation of LMTK- cells. The corresponding class I molecules reacted differentially with monomorphic monoclonal antibodies (m.Ab). Absorption and elution studies of the human alloantibodies reacting with the transformed cells and cellular radioimmunoassay of these cells with polymorphic m.Ab resulted in the identification of HLA-A3 and CW3 molecules. These transformed cells were used to immunize C3H mice and induce the production of xenogeneic antisera, which, following absorption, showed polymorphic reactivity with human cells, suggesting that some of these sera could be used as typing reagents.

Citing Articles

Cloning and sequencing the first HLA gene.

Jordan B Genetics. 2010; 184(4):879-86.

PMID: 20457890 PMC: 2865924. DOI: 10.1534/genetics.110.113852.

Transformation of murine LMTK- cells with purified HLA class I genes. III. Human HLA class I antigens coded by hybrid genes constructed in vitro indicate association of serologic reactivities with the first two domains of the molecule.

Jordan B, Lemonnier F, Caillol D, Trucy J Immunogenetics. 1983; 18(2):165-71.

PMID: 6885121 DOI: 10.1007/BF00368546.

Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: implications for the evolution of HLA genes.

Strachan T, Sodoyer R, Damotte M, Jordan B EMBO J. 1984; 3(4):887-94.

PMID: 6609814 PMC: 557443. DOI: 10.1002/j.1460-2075.1984.tb01901.x.

Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3).

Sodoyer R, Damotte M, Delovitch T, Trucy J, Jordan B, Strachan T EMBO J. 1984; 3(4):879-85.

PMID: 6609813 PMC: 557442. DOI: 10.1002/j.1460-2075.1984.tb01900.x.

Serological expression after sequential double transfection with purified HLA-A11 gene of mouse fibroblasts carrying human beta-2 microglobulin.

Paul P, Lepage V, Sayagh B, Metzger J, Pla M, Boumsell L Immunogenetics. 1985; 22(1):1-8.

PMID: 3894226 DOI: 10.1007/BF00430589.

References

Biddison W, Krangel M, Strominger J, Ward F, Shearer G, Shaw S . Virus-immune cytotoxic T cells recognize structural differences between serologically indistinguishable HLA-A2 molecules. Hum Immunol. 1980; 1(3):225-32. DOI: 10.1016/0198-8859(80)90017-8. View

Lemonnier F, Le Bouteiller P, Malissen B, Golstein P, Malissen M, Mishal Z . Transformation of murine LMTK- cells with purified HLA class I genes. I. Modification of conformation of murine beta 2-microglobulin upon its association with HLA heavy chains. J Immunol. 1983; 130(3):1432-8. View

Lemonnier F, Malissen M, Golstein P, Le Bouteiller P, Rebai N, Damotte M . Expression of human class I histocompatibility antigens at the surface of DNA-transformed mouse L cells. Immunogenetics. 1982; 16(4):355-61. DOI: 10.1007/BF00372307. View

Billing R, Clark B, Lucero K, Shi B, Terasaki P . A monoclonal antibody recognizing a determinant common to HLA-A3 and A11. Tissue Antigens. 1982; 19(2):121-8. DOI: 10.1111/j.1399-0039.1982.tb01427.x. View

Singer D, Camerini-Otero R, Satz M, Osborne B, Sachs D, Rudikoff S . Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells. Proc Natl Acad Sci U S A. 1982; 79(5):1403-7. PMC: 345981. DOI: 10.1073/pnas.79.5.1403. View

Dosseto M, Rohner C, Pierres M, Goridis C . Biosynthetic incorporation of [75Se]selenomethionine: a new method for labelling lymphocyte membrane antigens. J Immunol Methods. 1981; 41(2):145-53. DOI: 10.1016/0022-1759(81)90238-6. View

Oi V, Jones P, Goding J, Herzenberg L . Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978; 81:115-20. DOI: 10.1007/978-3-642-67448-8_18. View

Trucco M, Garotta G, Stocker J, Ceppellini R . Murine monoclonal antibodies against HLA structures. Immunol Rev. 1979; 47:219-52. DOI: 10.1111/j.1600-065x.1979.tb00295.x. View

Brodsky F, Parham P, Barnstable C, Crumpton M, Bodmer W . Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979; 47:3-61. DOI: 10.1111/j.1600-065x.1979.tb00288.x. View

10.

Malissen B, Charmot D, Mawas C . Expansion of human lymphocyte populations expressing specific immune reactivities. III. Specific colonies, either cytotoxic or proliferative, obtained from a population of responder cells primed in vitro. Preliminary immunogenetic analysis. Hum Immunol. 1981; 2(1):1-13. DOI: 10.1016/0198-8859(81)90002-1. View

11.

Rebai N, Malissen B, Pierres M, Accolla R, Corte G, Mawas C . Distinct HLA-DR epitopes and distinct families of HLA-Dr molecules defined by 15 monoclonal antibodies (mAb) either anti-DR or allo-anti-Iak cross-reacting with human DR molecule. I. Cross-inhibition studies of mAb cell surface fixation and.... Eur J Immunol. 1983; 13(2):106-11. DOI: 10.1002/eji.1830130205. View

12.

Malissen B, Kristensen T, Goridis C, Madsen M, Mawas C . Clones of human cytotoxic T lymphocytes derived from an allosensitized individual: HLA specificity and cell surface markers. Scand J Immunol. 1981; 14(3):213-24. DOI: 10.1111/j.1365-3083.1981.tb00558.x. View

13.

Barbosa J, Kamarck M, Biro P, Weissman S, Ruddle F . Identification of human genomic clones coding the major histocompatibility antigens HLA-a2 and HLA-B7 by DNA-mediated gene transfer. Proc Natl Acad Sci U S A. 1982; 79(20):6327-31. PMC: 347114. DOI: 10.1073/pnas.79.20.6327. View

14.

Wigler M, Silverstein S, Lee L, Pellicer A, Cheng Y, Axel R . Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell. 1977; 11(1):223-32. DOI: 10.1016/0092-8674(77)90333-6. View

15.

Barnstable C, Bodmer W, Brown G, Galfre G, Milstein C, Williams A . Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978; 14(1):9-20. DOI: 10.1016/0092-8674(78)90296-9. View

16.

Liabeuf A, le Borgne De Kaouel C, KOURILSKY F, Malissen B, MANUEL Y, Sanderson A . An antigenic determinant of human beta 2-microglobulin masked by the association with HLA heavy chains at the cell surface: analysis using monoclonal antibodies. J Immunol. 1981; 127(4):1542-8. View

17.

Malissen M, Damotte M, Birnbaum D, Trucy J, Jordan B . HLA cosmid clones show complete, widely spaced human class I genes with occasional clusters. Gene. 1982; 20(3):485-9. DOI: 10.1016/0378-1119(82)90219-0. View

18.

Brodsky F, Parham P . Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J Immunol. 1982; 128(1):129-35. View

19.

Shulman N, Marder V, Hiller M, COLLIER E . PLATELET AND LEUKOCYTE ISOANTIGENS AND THEIR ANTIBODIES: SEROLOGIC PHYSIOLOGIC AND CLINICAL STUDIES. Prog Hematol. 1964; 4:222-304. View

20.

Goding J . Use of staphylococcal protein A as an immunological reagent. J Immunol Methods. 1978; 20:241-53. DOI: 10.1016/0022-1759(78)90259-4. View