Identification of an Exposed Region of the Immunogenic Capsid Polypeptide VP1 on Foot-and-mouth Disease Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1983 Apr 1

PMID 6186823

Citations 17

Authors

B H Robertson

D M Moore

M J Grubman

D G Kleid

Affiliations

Soon will be listed here.

Abstract

Iodination of intact foot-and-mouth disease virus results in the selective labeling of VP1, substantiating its exposed location on the virion. A comparison of tryptic peptides revealed that a single tyrosine-containing peptide was labeled with iodine on intact or protease-cleaved virus. The labeled peptide from intact and protease-cleaved virus was characterized by molecular weight sizing and sequence analysis. Carboxypeptidase digestion of intact VP1, limited trypsin-cleaved VP1, and VP1 purified from bacterially contaminated tissue cultures yielded carboxyterminal residues of leucine, valine-arginine, and serine-alanine, respectively. The correlation of these findings with previous data on the amino acid sequence derived from nucleotide sequencing of serotypes A12 and O1 of foot-and-mouth disease virus VP1 places the probable exposed antigenic region of VP1 in a serotype-variable region including residues 136 through 144.

Citing Articles

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