Selective Amplification of Polymorphic Dihydrofolate Reductase Gene Loci in Chinese Hamster Lung Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Nov 1

PMID 6184719

Citations 12

Authors

J A Lewis

J P Davide

P W Melera

Affiliations

Soon will be listed here.

Abstract

A series of antifolate-resistant Chinese hamster lung sublines that overproduce either a Mr 20,000 or a Mr 21,000 class of dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3), known to contain amplified DHFR genes, has been analyzed by DNA and RNA transfer techniques. The results suggest that the Mr 20,000 and Mr 21,000 DHFRs are encoded by at least two polymorphic DHFR genes, both of which are expressed in drug-sensitive parental cells. In drug-resistant sublines only one of the two DHFR gene types is amplified, thus accounting for the overproduction of one or the other molecular weight class of DHFR. In addition to the known differences between the DHFRs whose overproduction they direct, these allelic genes differ in restriction endonuclease profiles and in the relative abundances of their multiple mRNA transcripts.

Citing Articles

A genetic polymorphism within the third poly(A) signal of the DHFR gene alters the polyadenylation pattern of DHFR transcripts in CHL cells.

Yang H, Melera P Nucleic Acids Res. 1994; 22(13):2694-702.

PMID: 8041633 PMC: 308229. DOI: 10.1093/nar/22.13.2694.

Specific DNA sequence amplification in human neuroblastoma cells.

Montgomery K, Biedler J, Spengler B, Melera P Proc Natl Acad Sci U S A. 1983; 80(18):5724-8.

PMID: 6577451 PMC: 384331. DOI: 10.1073/pnas.80.18.5724.

Expression of the amplified domain in human neuroblastoma cells.

Michitsch R, Montgomery K, Melera P Mol Cell Biol. 1984; 4(11):2370-80.

PMID: 6549047 PMC: 369067. DOI: 10.1128/mcb.4.11.2370-2380.1984.

Phenotypic expression in Escherichia coli and nucleotide sequence of two Chinese hamster lung cell cDNAs encoding different dihydrofolate reductases.

Melera P, Davide J, Hession C, Scotto K Mol Cell Biol. 1984; 4(1):38-48.

PMID: 6366511 PMC: 368655. DOI: 10.1128/mcb.4.1.38-48.1984.

Amplification of a cloned Chinese hamster dihydrofolate reductase gene after transfer into a dihydrofolate reductase-deficient cell line.

Milbrandt J, Azizkhan J, Hamlin J Mol Cell Biol. 1983; 3(7):1274-82.

PMID: 6310371 PMC: 370118. DOI: 10.1128/mcb.3.7.1274-1282.1983.

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