Continuous Growth of Proximal Tubular Kidney Epithelial Cells in Hormone-supplemented Serum-free Medium

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1982 Sep 1

PMID 6182146

Citations 26

Authors

L Chuman

L G Fine

A H Cohen

M H Saier Jr

Affiliations

Soon will be listed here.

Abstract

An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone-supplemented serum-free medium. Continuous growth requires the presence of seven factors: transferrin, insulin, selenium, hydrocortisone, triiodothyronine, vasopressin, and cholesterol. The hormone-defined medium (a) supports growth of LLC-PK1 cells at a rate of approaching that observed in serum-supplemented medium; (b) allows vectorial transepithelial salt and fluid transport as measured by hemicyst formation; and (c) influences cell morphology. The vasopressin dependency for growth and morphology can be partially replaced by isobutylmethylxanthine or dibutyryl cyclic AMP. The medium has been used to isolate rabbit proximal tubular kidney epithelial cells free of fibroblasts.

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References

GAUSH C, HARD W, Smith T . Characterization of an established line of canine kidney cells (MDCK). Proc Soc Exp Biol Med. 1966; 122(3):931-5. DOI: 10.3181/00379727-122-31293. View

LEIGHTON J, BRADA Z, Estes L, JUSTH G . Secretory activity and oncogenicity of a cell line (MDCK) derived from canine kidney. Science. 1969; 163(3866):472-3. DOI: 10.1126/science.163.3866.472. View

LEIGHTON J, Estes L, Mansukhani S, BRADA Z . A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium. Cancer. 1970; 26(5):1022-8. DOI: 10.1002/1097-0142(197011)26:5<1022::aid-cncr2820260509>3.0.co;2-m. View

Hayashi I, Sato G . Replacement of serum by hormones permits growth of cells in a defined medium. Nature. 1976; 259(5539):132-4. DOI: 10.1038/259132a0. View

Stiles C, Desmond W, Chuman L, Sato G, Saier M . Growth control of heterologous tissue culture cells in the congenitally athymic nude mouse. Cancer Res. 1976; 36(4):1353-60. View

Misfeldt D, Hamamoto S, Pitelka D . Transepithelial transport in cell culture. Proc Natl Acad Sci U S A. 1976; 73(4):1212-6. PMC: 430232. DOI: 10.1073/pnas.73.4.1212. View

Rindler M, Taub M, Saier Jr M . Uptake of 22Na+ by cultured dog kidney cells (MDCK). J Biol Chem. 1979; 254(22):11431-9. View

Taub M, Saier Jr M . Regulation of 22Na+ transport by calcium in an established kidney epithelial cell line. J Biol Chem. 1979; 254(22):11440-4. View

Sang U, Saier Jr M, Ellisman M . Tight junction formation is closely linked to the polar redistribution of intramembranous particles in aggregating MDCK epithelia. Exp Cell Res. 1979; 122(2):384-91. DOI: 10.1016/0014-4827(79)90315-x. View

10.

Rizzino A, Rizzino H, Sato G . Defined media and the determination of nutritional and hormonal requirements of mammalian cells in culture. Nutr Rev. 1979; 37(12):369-78. DOI: 10.1111/j.1753-4887.1979.tb06646.x. View

11.

Sang U, Saier Jr M, Ellisman M . Tight junction formation in the establishment of intramembranous particle polarity in aggregating MDCK cells. Effect of drug treatment. Exp Cell Res. 1980; 128(1):223-35. DOI: 10.1016/0014-4827(80)90406-1. View

12.

Rabito C, Ausiello D . Na+-dependent sugar transport in a cultured epithelial cell line from pig kidney. J Membr Biol. 1980; 54(1):31-8. DOI: 10.1007/BF01875374. View

13.

Saier Jr M . Growth and differentiated properties of a kidney epithelial cell line (MDCK). Am J Physiol. 1981; 240(3):C106-9. DOI: 10.1152/ajpcell.1981.240.3.C106. View

14.

BAR-KHAYIM Y, Cohen A, Houston B, Trizna W, Fine L . In vivo "culture" of a transporting renal epithelial monolayer. Kidney Int. 1981; 19(3):465-70. DOI: 10.1038/ki.1981.41. View

15.

Stiles C, Desmond W, Chuman L, Sato G, Saier Jr M . Relationship of cell growth behavior in vitro to tumorigenicity in athymic nude mice. Cancer Res. 1976; 36(9 pt.1):3300-5. View

16.

HULL R, CHERRY W, Weaver G . The origin and characteristics of a pig kidney cell strain, LLC-PK. In Vitro. 1976; 12(10):670-7. DOI: 10.1007/BF02797469. View

17.

Cereijido M, Robbins E, Dolan W, Rotunno C, Sabatini D . Polarized monolayers formed by epithelial cells on a permeable and translucent support. J Cell Biol. 1978; 77(3):853-80. PMC: 2110138. DOI: 10.1083/jcb.77.3.853. View

18.

Goldring S, Dayer J, Ausiello D, Krane S . A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressin. Biochem Biophys Res Commun. 1978; 83(2):434-40. DOI: 10.1016/0006-291x(78)91009-4. View

19.

Rabito C, Tchao R, Valentich J, LEIGHTON J . Distribution and characteristics of the occluding junctions in a monolayer of a cell line (MDCK) derived from canine kidney. J Membr Biol. 1978; 43(4):351-65. DOI: 10.1007/BF01871696. View

20.

Rindler M, Chuman L, Shaffer L, Saier Jr M . Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK). J Cell Biol. 1979; 81(3):635-48. PMC: 2110404. DOI: 10.1083/jcb.81.3.635. View