A Histological Method for the Visualization of the Intercellular Permeability Barrier in Mammalian Stratified Squamous Epithelia

Overview

Journal Histochem J

Specialty Biochemistry

Date 1982 Jul 1

PMID 6181022

Citations 3

Authors

M W Hill

C A Squier

J E Linder

Affiliations

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Abstract

Mammalian epidermis and oral epithelial possess an intercellular permeability barrier which is located in the superficial region of the tissue. This study reports a staining reaction which appears to demonstrate a histological correlate of this functional property. Specimens of ear skin, palate, buccal and oesophageal mucosa and of cornea and bladder were obtained from adult rabbits and rats, bisected and either incubated in vitro with 2.5% horseradish peroxidase as a tracer or fixed and processed for light microscopy and stained with a modification of Hart's elastin stain. Examination of specimens prepared by each procedure showed a complementary staining pattern in the intercellular spaces of the stratum corneum or in the superficial region of the non-keratinized tissue. In the epidermis and oral and oesophageal epithelia, the region which excluded the tracer stained with the modified elastin stain. In contrast, the corneal and bladder epithelia neither excluded the tracer nor showed intercellular staining. This relationship between staining of the intercellular space and the exclusion of tracer suggests that the intercellular material in the superficial region of epithelia may be chemically altered to form a barrier substance, possibly as the result of the discharge of the contents of the membrane-coating granules which are present in all the epithelia examined except the cornea and bladder.

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References

GRAHAM Jr R, Karnovsky M . The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966; 14(4):291-302. DOI: 10.1177/14.4.291. View

Hicks R . Hyperplasia and cornification of the transitional epithelium in the vitamin A-deficient rat. Changes in fine structure of the cells. J Ultrastruct Res. 1968; 22(3):206-30. DOI: 10.1016/s0022-5320(68)90016-6. View

Wolff K, Honigsmann H . Permeability of the epidermis and the phagocytic activity of keratinocytes. Ultrastructural studies with thorotrast as a marker. J Ultrastruct Res. 1971; 36(1):176-90. DOI: 10.1016/s0022-5320(71)80096-5. View

Mishima S, HEDBYS B . The permeability of the corneal epithelium and endothelium to water. Exp Eye Res. 1967; 6(1):10-32. DOI: 10.1016/s0014-4835(67)80049-6. View

Ashrafi S, Squier C, Meyer J . Staining of oral epithelium with the zinc iodide-osmium reaction. Histochem J. 1981; 13(1):45-55. DOI: 10.1007/BF01005838. View

Ross R . The elastic fiber. J Histochem Cytochem. 1973; 21(3):199-208. DOI: 10.1177/21.3.199. View

Squier C, Hopps R . A study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro. Br J Dermatol. 1976; 95(2):123-9. DOI: 10.1111/j.1365-2133.1976.tb00814.x. View

Squier C, Rooney L . The permeability of keratinized and nonkeratinized oral epithelium to lanthanum in vivo. J Ultrastruct Res. 1976; 54(2):286-95. DOI: 10.1016/s0022-5320(76)80157-8. View

Melcher A . Some histological and histochemical observations on the connective tissue of chronically inflamed human gingiva. J Periodontal Res. 1967; 2(2):127-46. DOI: 10.1111/j.1600-0765.1967.tb01881.x. View

10.

Hayward A . Electron microscopic observations on cell coat and membrane-coating granules of the epithelium of the hard and soft palate in the rat. Arch Oral Biol. 1973; 18(1):67-75. DOI: 10.1016/0003-9969(73)90021-6. View

11.

Bock J, Gebhart W . [Small granules in epithelial cells of human cornea (author's transl)]. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1973; 189(1):43-54. DOI: 10.1007/BF00411124. View

12.

Hill M, Squier C . The permeability of rat palatal mucosa maintained in organ culture. J Anat. 1979; 128(Pt 1):169-78. PMC: 1232970. View

13.

Squier C . The permeability of keratinized and nonkeratinized oral epithelium to horseradish peroxidase. J Ultrastruct Res. 1973; 43(1):160-77. DOI: 10.1016/s0022-5320(73)90076-2. View

14.

Elias P, Friend D . The permeability barrier in mammalian epidermis. J Cell Biol. 1975; 65(1):180-91. PMC: 2111161. DOI: 10.1083/jcb.65.1.180. View

15.

WISLOCKI G, Fawcett D, DEMPSEY E . Staining of stratified squamous epithelium of mucous membranes and skin of man and monkey by the periodic acid-Schiff method. Anat Rec. 1951; 110(3):359-75. DOI: 10.1002/ar.1091100307. View

16.

Hayward A . Membrane-coating granules. Int Rev Cytol. 1979; 59:97-127. DOI: 10.1016/s0074-7696(08)61661-7. View

17.

Elias P, GOERKE J, Friend D . Mammalian epidermal barrier layer lipids: composition and influence on structure. J Invest Dermatol. 1977; 69(6):535-46. DOI: 10.1111/1523-1747.ep12687968. View

18.

Schreiner E, Wolff K . [The permeability of the intercellular space of the epidermis for low molecular weight protein. Electron microscopic cytochemical studies with peroxidase as a tracer substance]. Arch Klin Exp Dermatol. 1969; 235(1):78-88. View