Suppression of BCG Cell Wall-induced Delayed-type Hypersensitivity by BCG Pre-treatment. II. Induction of Suppressor T Cells by Heat-killed BCG Injection

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1982 Apr 1

PMID 6175566

Citations 11

Authors

K Kato

K Yamamoto

Affiliations

Soon will be listed here.

Abstract

Previous intravenous (i.v.) injection of heat-killed Bacillus Calmette-Guérin (BCG) in mice produced a suppression of delayed-type hypersensitivity (DTH) induced with oil-treated BCG cell walls. This phenomenon was analysed by the macrophage migration inhibition (MI) test in which non-adherent spleen cells from mice which had been injected with heat-killed BCG (K-Non-ad. cells) were mixed with peritoneal exudate cells from BCG cell wall-immunized mice (effector-PEC). The test showed that the K-Non-ad. cells suppressed the MI activity in the effector-PEC, which indicated that the suppressor cells were induced in the spleen by the heat-killed BCG injections. Moreover, the suppressive effect of the K-Non-ad. cells disappeared after treatment with anti-brain associated theta serum (BA theta) and guinea-pig complement, and operated across the H-2 barrier. The suppressor cells inhibited the production or release of the migration inhibition factor from the BCG cell wall-sensitized cells in the presence of the specific antigen, purified protein derivatives (PPD). It was concluded that the injections of heat-killed BCG produced antigen non-specific suppressor T cells in DTH suppression.

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