Molecular Cloning and Analysis of CDNA Sequences Derived from Poly A+ RNA from Barley Endosperm: Identification of B Hordein Related Clones

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1981 Dec 21

PMID 6174944

Citations 41

Authors

B G Forde

M Kreis

M B Bahramian

J A Matthews

B J Miflin

R D Thompson

D Bartels

R B Flavell

Affiliations

Soon will be listed here.

Abstract

A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of 11 of the B hordein cDNA clones to Northern blots of size-fractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-c16, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to B1 hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.

Citing Articles

A broad view: Dick Flavell.

Zebell S Plant Physiol. 2021; 185(3):727-730.

PMID: 33822223 PMC: 8133605. DOI: 10.1093/plphys/kiaa111.

Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides.

de Vries S, Harmsen M, Kuiper M, Dons H, Wessels J Plant Mol Biol. 2013; 2(6):295-303.

PMID: 24318448 DOI: 10.1007/BF01578590.

Identification and DNA sequence analysis of a γ-type gliadin cDNA plasmid from winter wheat.

Okita T Plant Mol Biol. 2013; 3(6):325-32.

PMID: 24310564 DOI: 10.1007/BF00033378.

A simple and efficient procedure for isolating plant chromatin which is suitable for studies of DNase I-sensitive domains and hypersensitive sites.

Steinmuller K, Apel K Plant Mol Biol. 2013; 7(2):87-94.

PMID: 24302228 DOI: 10.1007/BF00040135.

Molecular cloning of two isoinhibitor forms of chymotrypsin inhibitor 1 (CI-1) from barley endosperm and their expression in normal and mutant barleys.

Williamson M, Forde J, Kreis M Plant Mol Biol. 2013; 10(6):521-35.

PMID: 24277624 DOI: 10.1007/BF00033607.

References

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Faulks A, Shewry P, Miflin B . The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hor-2 locus in barley (Hordeum vulgare L). Biochem Genet. 1981; 19(9-10):841-58. DOI: 10.1007/BF00504250. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Chamberlain J . Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979; 98(1):132-5. DOI: 10.1016/0003-2697(79)90716-4. View

Wienand U, Feix G . Electrophoretic fractionation and translation in vitro of poly(rA)-containing RNA from maize endosperm. Evidence of two mRNAs coding for zein protein. Eur J Biochem. 1978; 92(2):605-11. DOI: 10.1111/j.1432-1033.1978.tb12783.x. View

Clewell D . Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972; 110(2):667-76. PMC: 247463. DOI: 10.1128/jb.110.2.667-676.1972. View

Ohno T, Cozens P, Cato A, Jost J . Recombinant plasmids containing avian vitellogenin structural gene sequences derived from complementary DNA. Biochim Biophys Acta. 1980; 606(1):34-46. DOI: 10.1016/0005-2787(80)90095-7. View

Gerlach W, Bedbrook J . Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res. 1979; 7(7):1869-85. PMC: 342353. DOI: 10.1093/nar/7.7.1869. View

BAILEY J, Davidson N . Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976; 70(1):75-85. DOI: 10.1016/s0003-2697(76)80049-8. View

10.

Geiser M, Doring H, Wostemeyer J, Behrens U, Tillmann E, Starlinger P . A cDNA clone from Zea mays endosperm sucrose synthetase mRNA. Nucleic Acids Res. 1980; 8(24):6175-88. PMC: 328080. DOI: 10.1093/nar/8.24.6175. View

11.

Messing J, Crea R, Seeburg P . A system for shotgun DNA sequencing. Nucleic Acids Res. 1981; 9(2):309-21. PMC: 326694. DOI: 10.1093/nar/9.2.309. View

12.

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

13.

Alwine J, Kemp D, Parker B, Reiser J, Renart J, Stark G . Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979; 68:220-42. DOI: 10.1016/0076-6879(79)68017-5. View

14.

Peacock A, DINGMAN C . Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967; 6(6):1818-27. DOI: 10.1021/bi00858a033. View

15.

Ricciardi R, Miller J, Roberts B . Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proc Natl Acad Sci U S A. 1979; 76(10):4927-31. PMC: 413051. DOI: 10.1073/pnas.76.10.4927. View

16.

SANGER F, Coulson A . The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978; 87(1):107-10. DOI: 10.1016/0014-5793(78)80145-8. View

17.

Park W, Lewis E, Rubenstein I . Heterogeneity of zein mRNA and protein in maize. Plant Physiol. 1980; 65(1):98-106. PMC: 440275. DOI: 10.1104/pp.65.1.98. View

18.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

19.

Bishop J, Davies J . Plasmid cloning vectors that can be nicked at a unique site. Mol Gen Genet. 1980; 179(3):573-80. DOI: 10.1007/BF00271747. View

20.

Efstratiadis A, Kafatos F, Maxam A, Maniatis T . Enzymatic in vitro synthesis of globin genes. Cell. 1976; 7(2):279-88. DOI: 10.1016/0092-8674(76)90027-1. View