Molecular Weight of Human Gamma Interferon is Similar to That of Other Human Interferons

Overview

Journal Science

Specialty Science

Date 1982 Jan 22

PMID 6173921

Citations 13

Authors

Y K Yip

B S Barrowclough

C Urban

J Vilcek

Affiliations

Soon will be listed here.

Abstract

The molecular weight (as determined by molecular sieve chromatography) of human gamma interferon, formerly referred to as immune or type II interferon, is between 40,000 and 70,000. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gamma interferon activity was recovered mainly from two regions of the gels corresponding to molecular weights of 20,000 and 25,000. The results suggest that in native form human gamma interferon may be aggregated.

Citing Articles

Purification and characterization of interferon-like antiviral protein derived from flatfish (Paralichthys olivaceus) lymphocytes immortalized by oncogenes.

Tamai T, Shirahata S, Sato N, Kimura S, Nonaka M, Murakami H Cytotechnology. 1993; 11(2):121-31.

PMID: 7686026 DOI: 10.1007/BF00749001.

Necrosin: purification and properties of a cytotoxin derived from a murine macrophage-like cell line.

Kull Jr F, Cuatrecasas P Proc Natl Acad Sci U S A. 1984; 81(24):7932-6.

PMID: 6595667 PMC: 392267. DOI: 10.1073/pnas.81.24.7932.

Use of monoclonal antibodies as sensitive and specific probes for biologically active human gamma-interferon.

Chang T, McKinney S, Liu V, Kung P, Vilcek J, Le J Proc Natl Acad Sci U S A. 1984; 81(16):5219-22.

PMID: 6433346 PMC: 391669. DOI: 10.1073/pnas.81.16.5219.

Monoclonal antibodies against murine gamma interferon.

Prat M, Gribaudo G, Comoglio P, Cavallo G, Landolfo S Proc Natl Acad Sci U S A. 1984; 81(14):4515-9.

PMID: 6431408 PMC: 345621. DOI: 10.1073/pnas.81.14.4515.

Interrelationships of human interferon-gamma with lymphotoxin and monocyte cytotoxin.

Yip Y, Kelker H, Le J, Rubin B, Rinderknecht E, Aggarwal B J Exp Med. 1984; 159(3):828-43.

PMID: 6421983 PMC: 2187255. DOI: 10.1084/jem.159.3.828.