RNA Splicing Mutation in an Aberrantly Rearranged Immunoglobulin Lambda I Gene

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1981 Nov 1

PMID 6171827

Citations 17

Authors

N Hozumi

G E Wu

H Murialdo

L Roberts

D Vetter

W L Fife

M Whiteley

P Sadowski

Affiliations

Soon will be listed here.

Abstract

The mouse cell line MOPC 315 is an IgA (lambda II)-producing myeloma. We have studied a derivative of MOPC 315 that secretes normal lambda II chains but no heavy chain. This derivative, MOPC 315-26, was found to contain a rearranged lambda I gene in addition to a rearranged lambda II gene. The rearranged lambda I gene was cloned into bacteriophage lambda DNA and its structure was studied. The lambda I gene was found to have arisen by an aberrant recombination event that resulted in a single base insertion at the site of V-J region joining. In addition, the gene contained numerous point mutations in the vicinity of the junction of the V and J regions. Two point mutations occurred in the donor splice sequence normally used for the removal of the intron between the J and C regions, suggesting that the RNA synthesized from the aberrantly rearranged lambda I gene would be unable to undergo proper RNA splicing.

Citing Articles

Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.

Parslow T, Blair D, Murphy W, GRANNER D Proc Natl Acad Sci U S A. 1984; 81(9):2650-4.

PMID: 6425835 PMC: 345127. DOI: 10.1073/pnas.81.9.2650.

Immunoglobulin gene 'remnant' DNA--implications for antibody gene recombination.

Selsing E, Voss J, STORB U Nucleic Acids Res. 1984; 12(10):4229-46.

PMID: 6328416 PMC: 318828. DOI: 10.1093/nar/12.10.4229.

Site-specific recombination of yeast 2-micron DNA in vitro.

Vetter D, Andrews B, Sadowski P Proc Natl Acad Sci U S A. 1983; 80(23):7284-8.

PMID: 6316354 PMC: 390039. DOI: 10.1073/pnas.80.23.7284.

Nucleotide sequences of immunoglobulin mu heavy chain deletion mutants.

Baczynsky W, Sugii S, Murialdo H, Pennell N, Filkin C, Hozumi N Nucleic Acids Res. 1983; 11(21):7471-85.

PMID: 6316263 PMC: 326496. DOI: 10.1093/nar/11.21.7471.

Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells.

Ochi A, Hawley R, Hawley T, Shulman M, Traunecker A, Kohler G Proc Natl Acad Sci U S A. 1983; 80(20):6351-5.

PMID: 6312453 PMC: 394295. DOI: 10.1073/pnas.80.20.6351.

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