Modulation of Bacterial Association to HeLa Cell Cultures by Cell Density and by Chlamydial Infection

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1981 Oct 1

PMID 6170584

Citations 2

Authors

S K Bose

R L Mudd

Affiliations

Soon will be listed here.

Abstract

The effect of cell density on the rate of association of Escherichia coli and Staphylococcus aureus by monolayer cultures of HeLa 229 cells was investigated. Radioactively labeled bacteria were incubated with sparsely and densely plated cells. The rate of bacterial uptake was 10- to 20-fold higher in sparse cultures. Kinetic analysis of data with different multiplicities of input bacteria showed that the Km of the reaction was unaltered, whereas the Vmax was inversely related to cell density. Pretreatment of HeLa cultures with dibutyryl adenosine 3',5'-phosphate had little effect on the rate of bacterial association. The simultaneous presence of an obligately parasitic bacterium, Chlamydia trachomatis LGV434, enhanced the Vmax of association of E. coli and S. aureus. This effect was more pronounced in dense HeLa cell cultures. Heat-inactivated chlamydiae were unable to modify the association. Enhanced association persisted for at least 3 h after infection with chlamydiae.

Citing Articles

Influence of lectins, hexoses, and neuraminidase on the association of purified elementary bodies of Chlamydia trachomatis UW-31 with HeLa cells.

Bose S, Smith G, Paul R Infect Immun. 1983; 40(3):1060-7.

PMID: 6687878 PMC: 348158. DOI: 10.1128/iai.40.3.1060-1067.1983.

Interaction of chlamydiae and host cells in vitro.

MOULDER J Microbiol Rev. 1991; 55(1):143-90.

PMID: 2030670 PMC: 372804. DOI: 10.1128/mr.55.1.143-190.1991.

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