Stable Association of Viral Protein VP1 with Simian Virus 40 DNA

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1981 Aug 1

PMID 6168774

Citations 6

Authors

J N Brady

C A Lavialle

M F Radonovich

N P Salzman

Affiliations

Soon will be listed here.

Abstract

Mild dissociation of simian virus 40 particles releases a 110S virion core nucleoprotein complex containing histones and the three viral proteins VP1, VP2, and VP3. The association of viral protein VP1 within this nucleoprotein complex is mediated at least partially through a strong interaction with the viral DNA. Treatment of the virion-derived 110S nucleoprotein complex with 0.25% Sarkosyl dissociated VP2, VP3, and histones, leaving a stable VP1-DNA complex. The VP1-DNA complex had a sedimentation value of 30S and a density of 1.460 g/cm3. The calculated molecular weight of the complex was 7.9 x 10(6), with an average of 100 VP1 molecules per DNA. Agarose gel electrophoresis of the VP1-DNA complex demonstrated that VP1 is associated not only with form I and form II simian virus 40 DNAs but also with form III simian virus 40 DNA generated by cleavage with EcoRI.

Citing Articles

Salt-resistant association of simian virus 40 T antigen with simian virus 40 DNA in nucleoprotein complexes.

Rudolph K, Mann K J Virol. 1983; 47(2):276-86.

PMID: 6312066 PMC: 255259. DOI: 10.1128/JVI.47.2.276-286.1983.

Chemical cleavage of polyomavirus major structural protein VP1: identification of cleavage products and evidence that the receptor moiety resides in the carboxy-terminal region.

Anders D, Consigli R J Virol. 1983; 48(1):197-205.

PMID: 6310143 PMC: 255336. DOI: 10.1128/JVI.48.1.197-205.1983.

Simian virus 40 maturation: chromatin modifications increase the accessibility of viral DNA to nuclease and RNA polymerase.

Brady J, Radonovich M, Lavialle C, Salzman N J Virol. 1981; 39(2):603-11.

PMID: 6268846 PMC: 171370. DOI: 10.1128/JVI.39.2.603-611.1981.

DNA-binding properties of the major structural protein of simian virus 40.

Soussi T J Virol. 1986; 59(3):740-2.

PMID: 3016336 PMC: 253252. DOI: 10.1128/JVI.59.3.740-742.1986.

Analysis of temperature-sensitive mutations in the simian virus 40 gene encoding virion protein 1.

BEHM M, Lowman H, Ng S, Bina M Proc Natl Acad Sci U S A. 1988; 85(24):9421-5.

PMID: 2849104 PMC: 282764. DOI: 10.1073/pnas.85.24.9421.

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