Human Intestinal Mucosal Mast Cells: Evaluation of Fixation and Staining Techniques

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1981 Aug 1

PMID 6168659

Citations 86

Authors

S Strobel

H R Miller

A Ferguson

Affiliations

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Abstract

The staining properties of tissue mast cells are influenced by the method of fixation. Differences in fixation and staining techniques may explain the contradictory results in the published reports on the number of human mucosal mast cells (MMC) in the gastrointestinal mucosa in health and disease. We have examined the influence of fixatives on the staining properties of human MMC in operative biopsy specimens of human jejunum. Specimens were divided into pieces, each of which was fixed in one of the following fixatives: Carnoy's, basic lead acetate (BLA), Baker's, Bouin's, isotonic formol-acetic-acid (IFAA), 10% neutral buffered formalin, formol sublimate, and formol saline. Thereafter, tissues were paraffin-embedded and 5 micron sections were cut and stained with either astra-blue/safranin pH 0.3, or toluidine blue pH 0.5. Counts of the number of MMC/mm2 were obtained for each fixation method. The results show a critical influence of the fixative on the number of mast cells identified after staining. For example with astra-blue/safranin the mean MMC/mm2 count was 40 in formol-saline-fixed specimens, and 268 in Carnoy's-fixed specimens. In biopsies fixed with formalin-based fixatives, mast cells were more readily stained with toluidine blue. It is recommended that Carnoy's or BLA be used as the fixative for any light microscopic study of human MMC.

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References

Enerback L . Mast cells in rat gastrointestinal mucosa. 2. Dye-binding and metachromatic properties. Acta Pathol Microbiol Scand. 1966; 66(3):303-12. DOI: 10.1111/apm.1966.66.3.303. View

Miller H, Walshaw R . Immune reactions in mucous membranes. IV. Histochemistry of intestinal mast cells during helminth expulsion in the rat. Am J Pathol. 1972; 69(1):195-208. PMC: 2032783. View

Brandtzaeg P, Baklien K, Fausa O, Hoel P . Immunohistochemical characterization of local immunoglobulin formation in ulcerative colitis. Gastroenterology. 1974; 66(6):1123-36. View

Tas J, Berndsen R . Does heparin occur in mucosal mast cells of the rat small intestine?. J Histochem Cytochem. 1977; 25(9):1058-62. DOI: 10.1177/25.9.71326. View

Woodbury R, Gruzenski G, Lagunoff D . Immunofluorescent localization of a serine protease in rat small intestine. Proc Natl Acad Sci U S A. 1978; 75(6):2785-9. PMC: 392649. DOI: 10.1073/pnas.75.6.2785. View

Griscelli C, Vassalli P . The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978; 148(6):1661-77. PMC: 2185102. DOI: 10.1084/jem.148.6.1661. View

Batcup G, Spitz L . A histopathological study of gastric mucosal biopsies in infantile hypertrophic pyloric stenosis. J Clin Pathol. 1979; 32(6):625-8. PMC: 1145748. DOI: 10.1136/jcp.32.6.625. View

Sjolund K, Alumets J, BERG N, Hakanson R, Sundler F . Duodenal endocrine cells in adult coeliac disease. Gut. 1979; 20(7):547-52. PMC: 1412522. DOI: 10.1136/gut.20.7.547. View

Takahashi T, Shimazu H, Yamagishi T, Tani M . G-cell populations in resected stomachs from gastric and duodenal ulcer patients. Gastroenterology. 1980; 78(3):498-504. View

10.

Mayrhofer G . Thymus-dependent and thymus-independent subpopulations of intestinal intraepithelial lymphocytes: a granular subpopulation of probable bone marrow origin and relationship to mucosal mast cells. Blood. 1980; 55(3):532-5. View

11.

Piris J, Thomas N . A quantitative study of the influence of fixation on immunoperoxidase staining of rectal mucosal plasma cells. J Clin Pathol. 1980; 33(4):361-4. PMC: 1146079. DOI: 10.1136/jcp.33.4.361. View

12.

Selbekk B, Flaten O, Hanssen L . The in vitro effect of neurotensin on human jejunal mast cells. Scand J Gastroenterol. 1980; 15(4):457-60. DOI: 10.3109/00365528009181500. View

13.

DOLLBERG L, Gurevitz M, Freier S . Gastrointestinal mast cells in health, and in coeliac disease and other conditions. Arch Dis Child. 1980; 55(9):702-5. PMC: 1626996. DOI: 10.1136/adc.55.9.702. View

14.

Bloom G, Kelly J . The copper phthalocyanin dye "Astrablau" and its staining properties, especially the staining of mast cells. Z Zellforch Microsk Anat Histochem. 1960; 2:48-57. DOI: 10.1007/BF00736491. View