Physical Characterization of the IlvHI Operon of Escherichia Coli K-12

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Sep 1

PMID 6168634

Citations 31

Authors

C H Squires

M De Felice

S R Wessler

J M Calvo

Affiliations

Soon will be listed here.

Abstract

The ilvHI and leu genes of Escherichia coli K-12 are contained on a single 10.9-kilobase EcoRI fragment of deoxyribonucleic acid derived from the leu transducing phage lambda G4. Since the expression of all of these genes is controlled by leucine, we investigated whether they are part of single operon or whether they constitute separate but adjacent operons controlled from a common site. Both cloning and hybridization studies indicated that ilvHI and leu are distinct operons. They are transcribed in opposite directions and are separated by approximately 1,500 base pairs of deoxyribonucleic acid. Hybridization experiments showed that the expression of ilvHI is regulated chiefly at the level of transcription. The size of the ilvHI messenger ribonucleic acid is estimated to be 2,550 bases.

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References

Groves W, Davis Jr F, SELLS B . Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal Biochem. 1968; 22(2):195-210. DOI: 10.1016/0003-2697(68)90307-2. View

Calvo J, Freundlich M, Umbarger H . Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants. J Bacteriol. 1969; 97(3):1272-82. PMC: 249844. DOI: 10.1128/jb.97.3.1272-1282.1969. View

Guardiola J, De Felice M, Iaccarino M . Mutant of Escherichia coli K-12 missing acetolactate synthase activity. J Bacteriol. 1974; 120(1):536-8. PMC: 245796. DOI: 10.1128/jb.120.1.536-538.1974. View

Cooper T, Whitney P, MAGASANIK B . Reaction of lac-specific ribonucleic acid from Escherichia coli with lac deoxyribonucleic acid. J Biol Chem. 1974; 249(20):6548-55. View

De Felice M, Guardiola J, Malorni M, Klopotowski T, Iaccarino M . Regulation of the pool size of valine in Escherichia coli K-12. J Bacteriol. 1974; 120(3):1058-67. PMC: 245883. DOI: 10.1128/jb.120.3.1058-1067.1974. View

De Felice M, Guardiola J, Esposito B, Iaccarino M . Structural genes for a newly recognized acetolactate synthase in Escherichia coli K-12. J Bacteriol. 1974; 120(3):1068-77. PMC: 245884. DOI: 10.1128/jb.120.3.1068-1077.1974. View

Favre R, Wiater A, Puppo S, Iaccarino M . Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12. Mol Gen Genet. 1976; 143(3):243-52. DOI: 10.1007/BF00269400. View

BAILEY J, Davidson N . Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976; 70(1):75-85. DOI: 10.1016/s0003-2697(76)80049-8. View

Davis M, Calvo J . Isolation and characterization of lambda pleu bacteriophages. J Bacteriol. 1977; 129(2):1078-90. PMC: 235049. DOI: 10.1128/jb.129.2.1078-1090.1977. View

10.

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

11.

Davis M, Calvo J . Relationship between messenger ribonucleic acid and enzyme levels specified by the leucine operon of Escherichia coli K-12. J Bacteriol. 1977; 131(3):997-1007. PMC: 235558. DOI: 10.1128/jb.131.3.997-1007.1977. View

12.

De Felice M, Levinthal M . The acetohydroxy acid synthase III isoenzyme of Escherichia coli K-12: regulation of synthesis by leucine. Biochem Biophys Res Commun. 1977; 79(1):82-7. DOI: 10.1016/0006-291x(77)90063-8. View

13.

De Felice M, Squires C, Levinthal M, Guardiola J, Lamberti A, Iaccarino M . Growth inhibition of Escherichia coli K-12 by L-valine: a consequence of a regulatory pattern. Mol Gen Genet. 1977; 156(1):1-7. DOI: 10.1007/BF00272245. View

14.

Guardiola J, De Felice M, Lamberti A, Iaccarino M . The acetolactate synthase isoenzymes of Escherichia coli K-12. Mol Gen Genet. 1977; 156(1):17-25. DOI: 10.1007/BF00272247. View

15.

Alwine J, Kemp D, Stark G . Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977; 74(12):5350-4. PMC: 431715. DOI: 10.1073/pnas.74.12.5350. View

16.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

17.

Smith D, Calvo J . Regulation of dihydrofolate reductase synthesis in Escherichia coli. Mol Gen Genet. 1979; 175(1):31-8. DOI: 10.1007/BF00267852. View

18.

SUBRAHMANYAM C, McCorkle G, Umbarger H . Physical location of the ilvO determinant in Escherichia coli K-12 deoxyribonucleic acid. J Bacteriol. 1980; 142(2):547-55. PMC: 294022. DOI: 10.1128/jb.142.2.547-555.1980. View

19.

Bachmann B, Low K . Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980; 44(1):1-56. PMC: 373233. DOI: 10.1128/mr.44.1.1-56.1980. View

20.

Smith D, Calvo J . Nucleotide sequence of the E coli gene coding for dihydrofolate reductase. Nucleic Acids Res. 1980; 8(10):2255-74. PMC: 324076. DOI: 10.1093/nar/8.10.2255. View