A Human Choriocarcinoma Xenograft in Nude Mice; a Model for the Study of Antibody Localization

Overview

Journal Br J Cancer

Specialty Oncology

Date 1981 Aug 1

PMID 6168276

Citations 11

Authors

F Searle

J Boden

J C Lewis

K D Bagshawe

Affiliations

Soon will be listed here.

Abstract

The successful development of the concept of linking cell-killing agents to tumour-specific antibodies will be largely determined by the extent to which the antibodies are preferentially localized in the malignant tissue. A xenograft of human choriocarcinoma (CC3) has been established in nude mice, and the relative distribution of affinity-purified specific antibodies to human chorionic gonadotrophin has been compared with that of nonspecific antibodies from the same species. Treatment of the nonspecific antibodies with ammonium thiocyanate appeared to be important to ensure that the distributions in normal nude mice were equivalent. Specificity indices, derived from the comparative distributions of isotope activity in the tumour and lung of labelled specific and nonspecific antibodies, ranged between 1.3 and 2.0.

Citing Articles

Scanning for tumours with radiolabelled antibodies: a review.

Dykes P, Bradwell A, Fairweather D J R Soc Med. 1983; 76(11):957-60.

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Third Gordon Hamilton-Fairley Memorial Lecture. Tumour markers--where do we go from here?.

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PMID: 6192836 PMC: 2011455. DOI: 10.1038/bjc.1983.172.

The potential of carboxypeptidase G2-antibody conjugates as anti-tumour agents. I. Preparation of antihuman chorionic gonadotrophin-carboxypeptidase G2 and cytotoxicity of the conjugate against JAR choriocarcinoma cells in vitro.

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Distribution of intact and Fab2(1) fragments of anti-human chorionic gonadotrophin antibodies in nude mice bearing human choriocarcinoma xenografts.

Searle F, Adam T, Boden J Cancer Immunol Immunother. 1986; 21(3):205-8.

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Activity and distribution studies of etoposide and mitozolomide in vivo and in vitro against human choriocarcinoma cell lines.

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