Studies with Some Influenza B Viruses in Cell Cultures, Hamsters and Hamster Tracheal Organ Cultures

Overview

Journal Med Microbiol Immunol

Specialty Allergy & Immunology

Date 1981 Jan 1

PMID 6166842

Citations 5

Authors

P Reeve

M Pibermann

B Gerendas

Affiliations

Soon will be listed here.

Abstract

Six influenza B virus strains and one recombinant vaccine strain have been compared in cell cultures, hamsters and in hamster tracheal organ cultures. In cell cultures all strains plaque well with or without trypsin. All strains are restricted in growth above 38 degrees C. The cold adapted attenuated virus, influenza B/AA/1/66, and a cold recombinant, RB77, are also restricted in growth above 37 degrees C and thus have a temperature marker. In hamsters influenza B viruses, except strain B/Lee, grow well in lungs and nasal tissues. Small differences were seen between wild type viruses and the cold adapted irus and its recombinants which may serve as additional markers for attenuation.

Citing Articles

A comprehensive review of influenza B virus, its biological and clinical aspects.

Ashraf M, Asif Raza M, Amjad M, Din G, Yue L, Shen B Front Microbiol. 2024; 15:1467029.

PMID: 39296301 PMC: 11408344. DOI: 10.3389/fmicb.2024.1467029.

Comparative Pathology of Animal Models for Influenza A Virus Infection.

Kirk N, Liang Y, Ly H Pathogens. 2024; 13(1).

PMID: 38251342 PMC: 10820042. DOI: 10.3390/pathogens13010035.

Syrian Hamster as an Animal Model for the Study of Human Influenza Virus Infection.

Iwatsuki-Horimoto K, Nakajima N, Ichiko Y, Sakai-Tagawa Y, Noda T, Hasegawa H J Virol. 2017; 92(4).

PMID: 29212926 PMC: 5790951. DOI: 10.1128/JVI.01693-17.

Animal Models for Influenza Virus Pathogenesis and Transmission.

Bouvier N, Lowen A Viruses. 2011; 2(8):1530-1563.

PMID: 21442033 PMC: 3063653. DOI: 10.3390/v20801530.

Coinfection with influenza B virus does not affect association of Neisseria meningitidis with human nasopharyngeal mucosa in organ culture.

Read R, Goodwin L, Parsons M, Silcocks P, Kaczmarski E, Parker A Infect Immun. 1999; 67(6):3082-6.

PMID: 10338524 PMC: 96625. DOI: 10.1128/IAI.67.6.3082-3086.1999.

References

Sugiura A, Tobita K, KILBOURNE E . Isolation and preliminary characterization of temperature-sensitive mutants of influenza virus. J Virol. 1972; 10(4):639-47. PMC: 356514. DOI: 10.1128/JVI.10.4.639-647.1972. View

Beare A . Live viruses for immunization against influenza. Prog Med Virol. 1975; 20:49-83. View

Oxford J, McGeoch D, Schild G, Beare A . Analysis of virion RNA segments and polypeptides of influenza A virus recombinants of defined virulence. Nature. 1978; 273(5665):778-9. DOI: 10.1038/273778a0. View

Reeve P, Almond J, Felsenreich V, Pibermann M, Maassab H . Studies with a cold-recombinant A/Victoria/3/75 (H3N2) virus. I. biologic, genetic, and biochemical characterization. J Infect Dis. 1980; 142(6):850-6. DOI: 10.1093/infdis/142.6.850. View

Murphy B, Markoff L, Chanock R, Spring S, Maassab H, Kendal A . Genetic approaches to attenuation of influenza A viruses for man. Philos Trans R Soc Lond B Biol Sci. 1980; 288(1029):401-15. DOI: 10.1098/rstb.1980.0017. View

Maassab H . Biologic and immunologic characteristics of cold-adapted influenza virus. J Immunol. 1969; 102(3):728-32. View

Cox N, Maassab H, Kendal A . Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25 degrees between recent H3N2 and H1N1 epidemic.... Virology. 1979; 97(1):190-4. DOI: 10.1016/0042-6822(79)90386-6. View

Beare A, HALL T . Recombinant influenza-A viruses as live vaccines for man. Report to the Medical Research Council's Committee on Influenza and other Respiratory Virus Vaccines. Lancet. 1971; 2(7737):1271-3. DOI: 10.1016/s0140-6736(71)90597-6. View

Reeve P . Growth of some attenuated influenza A viruses in hamsters. Med Microbiol Immunol. 1978; 166(1-4):133-9. DOI: 10.1007/BF02121142. View

10.

Murphy B, Tierney E, Spring S, Chanock R . Temperature-sensitive mutants of influenza A virus. XI. Transfer of ts lesions in the Hong Kong/68-ts-1 [a] virus to the influenza A/Udorn/72 wild type. J Infect Dis. 1976; 134(6):577-84. DOI: 10.1093/infdis/134.6.577. View

11.

Almond J, Haymerle H, Felsenreich V, Reeve P . The structural and infected cell polypeptides of influenza B virus. J Gen Virol. 1979; 45(3):611-21. DOI: 10.1099/0022-1317-45-3-611. View

12.

Spring S, Nusinoff S, Tierney E, Richman D, Murphy B, Chanock R . Temperature-sensitive mutants of influenza. VIII. Genetic and biological characterization of TS mutants of influenza virus A (H3N2) and their assignment to complementation groups. Virology. 1975; 66(2):542-50. DOI: 10.1016/0042-6822(75)90226-3. View