Antigen-pulsed Macrophage Monolayers As Specific Immunoabsorbents: Selective Absorption of Murine T Cells Committed to Soluble Protein Antigen

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1981 Jul 1

PMID 6166549

Citations 2

Authors

W Y Langdon

P G Holt

G R Shellam

Affiliations

Soon will be listed here.

Abstract

Antigen-pulsed macrophage (MO) monolayers in petri dishes were successfully employed as immunoabsorbents, for both the depletion and enrichment of T cells which proliferated upon exposure to soluble ovalbumin (OA). This procedure was shown not to deplete T cells reactive to a second antigen, and similarly MO monolayers pulsed with other antigens failed to deplete the T-cell preparation of OA-reactive cells. Employing brief incubation with trypsin/ethylenediamine tetracetic acid (EDTA), up to 2 X 10(6) OA-binding T cells could be recovered from each petri dish.

Citing Articles

Single-cell studies on hapten-specific B cells: response to T-cell-dependent antigens.

Hebbard G, Pike B, Nossal G Proc Natl Acad Sci U S A. 1984; 81(8):2479-83.

PMID: 6232611 PMC: 345085. DOI: 10.1073/pnas.81.8.2479.

Functional characteristics of the veiled cells in afferent lymph from the rat intestine.

Mayrhofer G, Holt P, Papadimitriou J Immunology. 1986; 58(3):379-87.

PMID: 3525397 PMC: 1453468.

References

Wigzell H, Andersson B . Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells. J Exp Med. 1969; 129(1):23-36. PMC: 2138587. DOI: 10.1084/jem.129.1.23. View

Davie J, Paul W . Receptors on immunocompetent cells. I. Receptor specificity of cells participating in a cellular immune response. Cell Immunol. 1970; 1(4):404-18. DOI: 10.1016/0008-8749(70)90017-1. View

Julius M, Masuda T, Herzenberg L . Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter. Proc Natl Acad Sci U S A. 1972; 69(7):1934-8. PMC: 426835. DOI: 10.1073/pnas.69.7.1934. View

Rosenthal A, Shevach E . Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973; 138(5):1194-212. PMC: 2139433. DOI: 10.1084/jem.138.5.1194. View

Shevach E, Rosenthal A . Function of macrophages in antigen recognition by guinea pig T lymphocytes. II. Role of the macrophage in the regulation of genetic control of the immune response. J Exp Med. 1973; 138(5):1213-29. PMC: 2139446. DOI: 10.1084/jem.138.5.1213. View

Peck A, Bach F . A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented media. J Immunol Methods. 1973; 3(2):147-63. DOI: 10.1016/0022-1759(73)90030-6. View

Julius M, Simpson E, Herzenberg L . A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973; 3(10):645-9. DOI: 10.1002/eji.1830031011. View

Rubin B, Wigzell H . Lymphocyte proliferation in vitro induced by hapten autologous protein conjugates. I. A study on the class of lymphocytes responding in vitro and on the nature and specificity of their receptors. J Exp Med. 1974; 139(3):732-53. PMC: 2139556. DOI: 10.1084/jem.139.3.732. View

Haas W, Schrader J, SZENBERG A . A new, simple method for the preparation of lymphocytes bearing specific receptors. Eur J Immunol. 1974; 4(8):565-70. DOI: 10.1002/eji.1830040809. View

10.

Julius M, Herzenberg L . Isolation of antigen-binding cells from unprimed mice: demonstration of antibody-forming cell precursor activity and correlation between precursor and secreted antibody avidities. J Exp Med. 1974; 140(4):904-20. PMC: 2139630. DOI: 10.1084/jem.140.4.904. View

11.

Haas W, Layton J . Separation of antigen-specific lymphocytes. I. Enrichment of antigen-binding cells. J Exp Med. 1975; 141(5):1004-14. PMC: 2189776. DOI: 10.1084/jem.141.5.1004. View

12.

Ben-Sasson S, Paul W, Shevach E, Green I . In vitro selection and extended culture of antigen-specific T lymphocytes. I. Description of selection culture procedure and initial characterization of selected cells. J Exp Med. 1975; 142(1):90-105. PMC: 2189876. DOI: 10.1084/jem.142.1.90. View

13.

Tada T, Taniguchi M, Takemori T . Properties of primed suppressor T cells and their products. Transplant Rev. 1975; 26:106-29. DOI: 10.1111/j.1600-065x.1975.tb00177.x. View

14.

Lipscomb M, Ben-Sasson S, UHR J . Specific binding of T lymphocytes to macrophages. I. Kinetics of binding. J Immunol. 1977; 118(5):1748-54. View

15.

Wing E, Remington J . Studies on the regulation of lymphocyte reactivity by normal and activated macrophages. Cell Immunol. 1977; 30(1):108-21. DOI: 10.1016/0008-8749(77)90052-1. View

16.

Braendstrup O, Werdelin O . Macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. IV. Synergy of lymphocytes in the formation of clusters. Cell Immunol. 1977; 32(2):263-73. DOI: 10.1016/0008-8749(77)90203-9. View

17.

Wood P, Simes R, Nelson D . Activity of mouse macrophages purified by adherence to, and removal from, a plastic surface. J Immunol Methods. 1979; 28(1-2):117-24. DOI: 10.1016/0022-1759(79)90333-8. View

18.

Werdelin O, Braendstrup O, Shevach E . Specific absorption of T lymphocytes committed to soluble protein antigens by incubation on antigen-pulsed macrophage monolayers. J Immunol. 1979; 123(4):1755-62. View

19.

Suzuki K, TOMASI Jr T . The regulatory effect of macrophages on the antigen-induced lymph node cell proliferative response. Cell Immunol. 1980; 49(2):317-28. DOI: 10.1016/0008-8749(80)90033-7. View

20.

Warner L, Holt P, Mayrhofer G . Alveolar macrophages. VI. Regulation of alveolar macrophage-mediated suppression of lymphocyte proliferation by a putative T cell. Immunology. 1981; 42(1):137-47. PMC: 1458214. View