Epstein-Barr Virus RNA. VI. Viral RNA in Restringently and Abortively Infected Raji Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1981 May 1

PMID 6165836

Citations 31

Authors

W King

V van Santen

E Kieff

Affiliations

Soon will be listed here.

Abstract

Nuclear and polyadenylated RNA fractions of Raji cells are encoded by larger fractions of Epstein-Barr virus DNA (35 and 18%, respectively) than encode polyribosomal RNA (10%). Polyribosomal RNA is encoded by DNA mapping at 0.05 X 10(8) to 0.29 X 10(8), 0.63 X 10(8) to 0.66 X 10(8), and 1.10 X 10(8) to 0.03 X 10(8) daltons. An abundant, small (160-base), non-polyadenylated RNA encoded by EcoRI fragment J (0.05 X 10(8) to 0.07 X 10(8) daltons) is also present in the cytoplasm of Raji cells. After induction of early antigen in Raji cells, there was a substantial increase in the complexity of viral polyadenylated and polyribosomal RNAs. Thus, nuclear RNA was encoded by 40% of Epstein-Barr virus DNA, and polyadenylated and polyribosomal RNAs were encoded by at least 30% of Epstein-Barr virus DNA. Polyribosomal RNA from induced Raji cells was encoded by Epstein-Barr virus DNAs mapping at 0.05 X 10(8) to 0.29 X 10(8), 0.63 X 10(8) to 0.66 X 10(8), and 1.10 X 10(8) to 0.03 X 10(8) daltons and also by DNAs mapping within the long unique regions of Epstein-Barr virus DNA at 0.39 X 10(8) to 0.49 X 10(8), 0.51 X 10(8) to 0.59 X 10(8), 0.66 X 10(8) to 0.77 X 10(8), and 1.02 X 10(8) to 1.05 X 10(8) daltons.

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References

Henle W, Henle G, Zajac B, Pearson G, Waubke R, Scriba M . Differential reactivity of human serums with early antigens induced by Epstein-Barr virus. Science. 1970; 169(3941):188-90. DOI: 10.1126/science.169.3941.188. View

Given D, Yee D, Griem K, Kieff E . DNA of Epstein-Barr virus. V. Direct repeats of the ends of Epstein-Barr virus DNA. J Virol. 1979; 30(3):852-62. PMC: 353396. DOI: 10.1128/JVI.30.3.852-862.1979. View

Gerber P . Activation of Epstein-Barr virus by 5-bromodeoxyuridine in "virus-free" human cells (complement-fixing antigen-immunofluorescence-leukocytes). Proc Natl Acad Sci U S A. 1972; 69(1):83-5. PMC: 427549. DOI: 10.1073/pnas.69.1.83. View

Miller G, Shope T, LISCO H, Stitt D, Lipman M . Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc Natl Acad Sci U S A. 1972; 69(2):383-7. PMC: 426463. DOI: 10.1073/pnas.69.2.383. View

Hayward G, Smith M . The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972; 63(3):383-95. DOI: 10.1016/0022-2836(72)90435-4. View

Nonoyama M, Pagano J . Separation of Epstein-Barr virus DNA from large chromosomal DNA in non-virus-producing cells. Nat New Biol. 1972; 238(84):169-71. DOI: 10.1038/newbio238169a0. View

Frenkel N, Roizman B . Ribonucleic acid synthesis in cells infected with herpes simplex virus: controls of transcription and of RNA abundance. Proc Natl Acad Sci U S A. 1972; 69(9):2654-8. PMC: 427009. DOI: 10.1073/pnas.69.9.2654. View

Sugawara K, OSATO T . Two distinct antigenic components in an Epstein-Barr virus-related early product induced by halogenated pyrimidines in non-producing human lymphoblastoid cells. Nat New Biol. 1973; 243(128):209-10. DOI: 10.1038/newbio243209a0. View

Hutton J, Wetmur J . Renaturation of bacteriophage phiX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant. J Mol Biol. 1973; 77(4):495-500. DOI: 10.1016/0022-2836(73)90218-0. View

10.

Given D, Kieff E . DNA of Epstein-Barr virus. VI. Mapping of the internal tandem reiteration. J Virol. 1979; 31(2):315-24. PMC: 353453. DOI: 10.1128/JVI.31.2.315-324.1979. View

11.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

12.

Berger S, Birkenmeier C . Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes. Biochemistry. 1979; 18(23):5143-9. DOI: 10.1021/bi00590a018. View

13.

Kieff E, Given D, Powell A, King W, Dambaugh T, Raab-Traub N . Epstein-Barr virus: structure of the viral DNA and analysis of viral RNA in infected cells. Biochim Biophys Acta. 1979; 560(3):355-73. DOI: 10.1016/0304-419x(79)90009-x. View

14.

Kallin B, Luka J, Klein G . Immunochemical characterization of Epstein-Barr virus-associated early and late antigens in n-butyrate-treated P3HR-1 cells. J Virol. 1979; 32(3):710-6. PMC: 525917. DOI: 10.1128/JVI.32.3.710-716.1979. View

15.

Lerner M, Boyle J, Mount S, Wolin S, Steitz J . Are snRNPs involved in splicing?. Nature. 1980; 283(5743):220-4. DOI: 10.1038/283220a0. View

16.

Hudewentz J, Bornkamm G, zur Hausen H . Effect of the diterpene ester TPA on Epstein-Barr virus antigen- and DNA synthesis in producer and nonproducer cell lines. Virology. 1980; 100(1):175-8. DOI: 10.1016/0042-6822(80)90563-2. View

17.

Rymo L . Identification of transcribed regions of Epstein-Barr virus DNA in Burkitt lymphoma-derived cells. J Virol. 1979; 32(1):8-18. PMC: 353521. DOI: 10.1128/JVI.32.1.8-18.1979. View

18.

Mueller-Lantzsch N, Georg B, Yamamoto N, zur Hausen H . Epstein-Barr virus-induced proteins. III. Analysis of polypeptides from P3HR-1-EBV-superinfected NC37 cells by immunoprecipitation. Virology. 1980; 102(1):231-3. DOI: 10.1016/0042-6822(80)90087-2. View

19.

Rogers J, Wall R . A mechanism for RNA splicing. Proc Natl Acad Sci U S A. 1980; 77(4):1877-9. PMC: 348611. DOI: 10.1073/pnas.77.4.1877. View

20.

Dambaugh T, Beisel C, Hummel M, King W, Fennewald S, Cheung A . Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping. Proc Natl Acad Sci U S A. 1980; 77(5):2999-3003. PMC: 349534. DOI: 10.1073/pnas.77.5.2999. View