Virus-coded DNA Endonuclease from Avian Retrovirus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1981 May 1

PMID 6165835

Citations 12

Authors

M Golomb

D P Grandgenett

W Mason

Affiliations

Soon will be listed here.

Abstract

Reverse transcriptase from avian retrovirus has a physically associated DNA endonuclease with novel substrate and cofactor requirements. A similar endonuclease activity copurifies with pp32, a protein from viral cores that has been identified with the non-alpha region of the beta subunit of reverse transcriptase. Several temperature-sensitive mutants of avian retrovirus with thermolabile DNA polymerase were tested for thermal sensitivity of their DNA endonuclease activity. Two pol mutants of Rous sarcoma virus, ts335 and ts337, had thermolabile DNA endonuclease; a temperature-resistant revertant of ts335 had a heat-stable DNA endonuclease. DNA endonuclease is therefore a product of the pol gene and an integral part of the reverse transcriptase. A second class of pol mutants, typified by ts568 and ts553, had thermolabile DNA polymerase, but heat-stable DNA endonuclease.

Citing Articles

Requirement for integrase during reverse transcription of human immunodeficiency virus type 1 and the effect of cysteine mutations of integrase on its interactions with reverse transcriptase.

Zhu K, Dobard C, Chow S J Virol. 2004; 78(10):5045-55.

PMID: 15113886 PMC: 400327. DOI: 10.1128/jvi.78.10.5045-5055.2004.

Retroviral DNA integration.

Hindmarsh P, Leis J Microbiol Mol Biol Rev. 1999; 63(4):836-43, table of contents.

PMID: 10585967 PMC: 98978. DOI: 10.1128/MMBR.63.4.836-843.1999.

Avian retrovirus pp32 DNA-binding protein. I. Recognition of specific sequences on retrovirus DNA terminal repeats.

Misra T, Grandgenett D, Parsons J J Virol. 1982; 44(1):330-43.

PMID: 6292495 PMC: 256268. DOI: 10.1128/JVI.44.1.330-343.1982.

A mutant murine leukemia virus with a single missense codon in pol is defective in a function affecting integration.

Donehower L, Varmus H Proc Natl Acad Sci U S A. 1984; 81(20):6461-5.

PMID: 6208550 PMC: 391944. DOI: 10.1073/pnas.81.20.6461.

Mechanism of action of the endonuclease associated with the alpha beta and beta beta forms of avian RNA tumor virus reverse transcriptase.

Leis J, Duyk G, Johnson S, Longiaru M, Skalka A J Virol. 1983; 45(2):727-39.

PMID: 6187936 PMC: 256468. DOI: 10.1128/JVI.45.2.727-739.1983.

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