Partial Purification and Characterization of Human Gamma (immune) Interferon

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1981 Mar 1

PMID 6165014

Citations 28

Authors

Y K Yip

R H Pang

C Urban

J Vilcek

Affiliations

Soon will be listed here.

Abstract

Human gamma (immune) interferon (IFN-gamma) was produced in lymphocyte cultures stimulated with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate) and purified phytohemagglutinin. Physicochemical analysis showed that human IFN-gamma is a glycoprotein with an isoelectric point around 8.6 and an apparent molecular weight of 58,000 +/- 3000. A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. This purification process resulted in an increase in specific activity from about 10(4) (crude culture fluid) to an estimated 10(7) units per mg of protein with a cumulative recovery of about 40% of the IFN activity.

Citing Articles

Interferon-gamma released from omental adipose tissue of insulin-resistant humans alters adipocyte phenotype and impairs response to insulin and adiponectin release.

Wentworth J, Zhang J, Bandala-Sanchez E, Naselli G, Liu R, Ritchie M Int J Obes (Lond). 2017; 41(12):1782-1789.

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Monoclonal antibodies to human interferon-gamma: production, affinity purification and radioimmunoassay.

Novick D, Eshhar Z, Fischer D, FRIEDLANDER J, Rubinstein M EMBO J. 1983; 2(9):1527-30.

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Radiation inactivation of human gamma-interferon: cellular activation requires two dimers.

Langer J, Rashidbaigi A, Garotta G, KEMPNER E Proc Natl Acad Sci U S A. 1994; 91(13):5818-22.

PMID: 8016072 PMC: 44088. DOI: 10.1073/pnas.91.13.5818.

Biosynthetic human interferon treatment of herpetic keratitis.

Smolin G Trans Am Ophthalmol Soc. 1983; 81:785-824.

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Interrelationships of human interferon-gamma with lymphotoxin and monocyte cytotoxin.

Yip Y, Kelker H, Le J, Rubin B, Rinderknecht E, Aggarwal B J Exp Med. 1984; 159(3):828-43.

PMID: 6421983 PMC: 2187255. DOI: 10.1084/jem.159.3.828.

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