Response of EMT6 Multicellular Tumour Spheroids to Hyperthermia and Cytotoxic Drugs

Overview

Journal Br J Cancer

Specialty Oncology

Date 1981 Mar 1

PMID 6164381

Citations 5

Authors

J E Morgan

N M Bleehen

Affiliations

Soon will be listed here.

Abstract

The response of multicellular tumour spheroids of the EMT6 cell line to combinations of hyperthermia and Bleomycin (BLM) or Adriamycin (ADM) has been investigated. Using this model system, we have demonstrated enhanced BLM cytotoxicity at 43 degrees C and also heat-induced drug tolerance to BLM at 43 degrees C. ADM cytotoxicity was not significantly increased after 43 degrees C x 1 h but after 6 h at 42 degrees C greatly enhanced cell-killing was evident. These results are discussed in relation to our previous data for EMT6 cells growing either as monolayer cultures in vitro or as solid tumours in mice.

Citing Articles

Effect of pH and moderate hyperthermia on doxorubicin, epirubicin and aclacinomycin A cytotoxicity for Chinese hamster ovary cells.

Kleeberger L, Rottinger E Cancer Chemother Pharmacol. 1993; 33(2):144-8.

PMID: 8261573 DOI: 10.1007/BF00685332.

Enhancement of thermal killing by polyamines V. The response of EMT6 multicellular tumour spheroids versus monolayer cells.

Ben-Hur E, Shaw J, Bleehen N Br J Cancer. 1983; 47(1):51-5.

PMID: 6821633 PMC: 2011250. DOI: 10.1038/bjc.1983.6.

Multicellular spheroids. A review on cellular aggregates in cancer research.

Mueller-Klieser W J Cancer Res Clin Oncol. 1987; 113(2):101-22.

PMID: 3549738 DOI: 10.1007/BF00391431.

Hyperthermia in cancer therapy.

Otte J Eur J Pediatr. 1988; 147(6):560-9.

PMID: 3053187 DOI: 10.1007/BF00442463.

A comparison of adriamycin and mAMSA. II. Studies with V79 and human tumour multicellular spheroids.

West C, Stratford I Cancer Chemother Pharmacol. 1987; 20(2):109-14.

PMID: 2822273 DOI: 10.1007/BF00253963.

References

Sutherland R, Durand R . Hypoxic cells in an in vitro tumour model. Int J Radiat Biol Relat Stud Phys Chem Med. 1973; 23(3):235-46. DOI: 10.1080/09553007314550261. View

Hahn G, Braun J . Thermochemotherapy: synergism between hyperthermia (42-43 degrees) and adriamycin (of bleomycin) in mammalian cell inactivation. Proc Natl Acad Sci U S A. 1975; 72(3):937-40. PMC: 432437. DOI: 10.1073/pnas.72.3.937. View

Sutherland R, Durand R . Radiation response of multicell spheroids--an in vitro tumour model. Curr Top Radiat Res Q. 1976; 11(1):87-139. View

Hahn G, Strande D . Cytotoxic effects of hyperthermia and adriamycin on Chinese hamster cells. J Natl Cancer Inst. 1976; 57(5):1063-7. DOI: 10.1093/jnci/57.5.1063. View

Dertinger H . Kinetic response of an in vitro "tumour-model" (V 79 spheroids) to 42 degrees C hyperthermia. Eur J Cancer (1965). 1977; 13(1):23-8. DOI: 10.1016/0014-2964(77)90225-0. View

Bleehen N, Honess D, Morgan J . Interaction of hyperthermia and the hypoxic cell sensitizer Ro-07-0582 on the EMT6 mouse tumour. Br J Cancer. 1977; 35(3):299-306. PMC: 2025289. DOI: 10.1038/bjc.1977.43. View

Sridhar R, Sutherland R . Hyperthermic potentiation of cytotoxicity of Ro-07-0582 in multicell spheroids. Int J Radiat Oncol Biol Phys. 1977; 2(5-6):531-5. DOI: 10.1016/0360-3016(77)90164-x. View

Yuhas J, Li A, Martinez A, LADMAN A . A simplified method for production and growth of multicellular tumor spheroids. Cancer Res. 1977; 37(10):3639-43. View

Yatvin M . The influence of membrane lipid composition and procaine on hyperthermic death of cells. Int J Radiat Biol Relat Stud Phys Chem Med. 1977; 32(6):513-21. DOI: 10.1080/09553007714551301. View

10.

Morgan J, Honess D, Bleehen N . The interaction of thermal tolerance with drug cytotoxicity in vitro. Br J Cancer. 1979; 39(4):422-8. PMC: 2009926. DOI: 10.1038/bjc.1979.75. View

11.

FIELD S, Bleehen N . Hyperthermia in the treatment of cancer. Cancer Treat Rev. 1979; 6(2):63-94. DOI: 10.1016/s0305-7372(79)80043-2. View

12.

Sutherland R, EDDY H, Bareham B, Reich K, VanAntwerp D . Resistance to adriamycin in multicellular spheroids. Int J Radiat Oncol Biol Phys. 1979; 5(8):1225-30. DOI: 10.1016/0360-3016(79)90643-6. View

13.

Meyn R, Corry P, Fletcher S, Demetriades M . Thermal enhancement of DNA strand breakage in mammalian cells treated with bleomycin. Int J Radiat Oncol Biol Phys. 1979; 5(9):1487-9. DOI: 10.1016/0360-3016(79)90755-7. View

14.

Twentyman P . Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. Br J Cancer. 1980; 42(2):297-304. PMC: 2010392. DOI: 10.1038/bjc.1980.230. View