In Vitro Translation of Maize ADH: Evidence for the Anaerobic Induction of MRNA

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1980 Aug 1

PMID 6162449

Citations 22

Authors

R J Ferl

M D Brennan

D Schwartz

Affiliations

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Abstract

Total cellular RNA from anaerobically stressed maize seedling roots was used to stimulate in vitro translation of authentic maize alcohol dehydrogenase (ADH) in a rabbit reticulocyte lysate system. Total products from such reactions were displayed on NEPHGE-SDS two-dimensional gels and the Adhl-specific translation products were identified by using RNA from sib seedlings segregating for Adhl charge and size variants. The application of a rapid RNA isolation procedure allowed the efficient isolation of biologically active RNAs from small amonts of seedling material. Maize ADHs translated in vitro are identical in size to in vivo ADH. Further, no ADH was detected in the products of an in vitro translation reaction stimulated by total RNA from aerobically grown seedlings. This suggests that induction of ADH protein by anaerobic stress is accomplished by production of Adh mRNA rather than activation of sequestered mRNA. The mRNAs for maize ADH1 and ADH2 are among a small class of mRNAs induced during anaerobiosis.

Citing Articles

Expression of alcohol dehydrogenase in rice embryos under anoxia.

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ARF-B(2): A Protein Complex that Specifically Binds to Part of the Anaerobic Response Element of Maize Adh 1.

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Hypoxic Induction of Anoxia Tolerance in Root Tips of Zea mays.

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Metabolic Acclimation to Anoxia Induced by Low (2-4 kPa Partial Pressure) Oxygen Pretreatment (Hypoxia) in Root Tips of Zea mays.

Saglio P, Drew M, Pradet A Plant Physiol. 1988; 86(1):61-6.

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Adaptation of ribonucleic Acid metabolism to anoxia in rice embryos.

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