Le Interferon MRNA from Human Fibroblasts

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 Sep 1

PMID 6159644

Citations 4

Authors

R H Pang

T G Hayes

J Vilcek

Affiliations

Soon will be listed here.

Abstract

Human F and Le interferon can be clearly distinguished on the basis of different antigenic properties and host range. After inoculation with Newcastle disease virus (NDV), GM-258 fibroblasts produced Le as well as F interferon; in contrast, only F interferon was detectable after stimulation with poly(I) . poly(C). Polyadenylylated mRNA isolated from fibroblasts induced with poly(I) . poly(C) or NDV was injected into Xenopus laevis oocytes and the interferon activities thus produced were analyzed. Only F interferon production was demonstrable in oocytes injected with mRNA from cells induced with poly(I) . poly(C), whereas both F and Le interferons were made in oocytes injected with mRNA from NDV-induced cultures. The time course of accumulation of F and Le interferon mRNAs in NDV-induced cells corresponded to the kinetics of F and Le interferon synthesis in intact cells. The ratio of F and Le interferons made in oocytes was similar to that observed in intact GM-258 cells. F and Le interferon mRNA activities isolated from GM-258 cells could not be separated by sucrose density gradient centrifugation. However, the profile of F mRNA activity was more heterogeneous and its peak sedimented somewhat more slowly than that of Le interferon mRNA. These results suggest that the varying ratios of F and Le interferon synthesis in different cells after different modes of stimulation are determined at the level of mRNA. The induction mechanisms of F and Le interferon mRNA synthesis appear to be closely related but not identical.

Citing Articles

Structure of a chromosomal gene for human interferon beta.

Ohno S, Taniguchi T Proc Natl Acad Sci U S A. 1981; 78(9):5305-9.

PMID: 16593086 PMC: 348733. DOI: 10.1073/pnas.78.9.5305.

Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells.

Content J, De Wit L, Tavernier J, Fiers W Nucleic Acids Res. 1983; 11(9):2627-38.

PMID: 6856471 PMC: 325913. DOI: 10.1093/nar/11.9.2627.

Inhibition of interferon production in human fibroblasts by a tumor promoting phorbol ester.

Frankfort H, Vilcek J Arch Virol. 1982; 73(3-4):295-309.

PMID: 6184036 DOI: 10.1007/BF01318083.

Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.

Taniguchi T, Pang R, Yip Y, Henriksen D, Vilcek J Proc Natl Acad Sci U S A. 1981; 78(6):3469-72.

PMID: 6167986 PMC: 319590. DOI: 10.1073/pnas.78.6.3469.

References

De Maeyer-Guignard J, De Maeyer E, Montagnier L . Interferon messenger RNA: translation in heterologous cells. Proc Natl Acad Sci U S A. 1972; 69(5):1203-7. PMC: 426664. DOI: 10.1073/pnas.69.5.1203. View

Vilcek J, Havell E . Stabilization of interferon messenger RNA activity by treatment of cells with metabolic inhibitors and lowering of the incubation temperature. Proc Natl Acad Sci U S A. 1973; 70(12):3909-13. PMC: 427355. DOI: 10.1073/pnas.70.12.3909. View

Tan Y, Creagan R, Ruddle F . The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5. Proc Natl Acad Sci U S A. 1974; 71(6):2251-5. PMC: 388429. DOI: 10.1073/pnas.71.6.2251. View

Havell E, Vilcek J, FALCOFF E, Berman B . Suppression of human interferon production by inhibitors of glycosylation. Virology. 1975; 63(2):475-83. DOI: 10.1016/0042-6822(75)90320-7. View

Berg K, OGBURN C, Paucker K, Mogensen K, Cantell K . Affinity chromatography of human leukocyte and diploid cell interferons on sepharose-bound antibodies. J Immunol. 1975; 114(2 Pt 1):640-4. View

Havell E, Berman B, OGBURN C, Berg K, Paucker K, Vilcek J . Two antigenically distinct species of human interferon. Proc Natl Acad Sci U S A. 1975; 72(6):2185-7. PMC: 432721. DOI: 10.1073/pnas.72.6.2185. View

Pestka S, McInnes J, Havell E, Vilcek J . Cell-free synthesis of human interferon. Proc Natl Acad Sci U S A. 1975; 72(10):3898-901. PMC: 433103. DOI: 10.1073/pnas.72.10.3898. View

Pang R, Phillips L . Nucleotide sequences in the RNA of mammalian leukemia and sarcoma viruses. Biochem Biophys Res Commun. 1975; 67(2):508-17. DOI: 10.1016/0006-291x(75)90841-4. View

Reynolds Jr F, Premkumar E, Pitha P . Interferon activity produced by translation of human interferon messenger RNA in cell-free ribosomal systems and in Xenopus oöcytes. Proc Natl Acad Sci U S A. 1975; 72(12):4881-5. PMC: 388836. DOI: 10.1073/pnas.72.12.4881. View

10.

Cavalieri R, Havell E, Vilcek J, Pestka S . Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells. Proc Natl Acad Sci U S A. 1977; 74(8):3287-91. PMC: 431533. DOI: 10.1073/pnas.74.8.3287. View

11.

Sehgal P, Dobberstein B, Tamm I . Interferon messenger RNA content of human fibroblasts during induction, shutoff, and superinduction of interferon production. Proc Natl Acad Sci U S A. 1977; 74(8):3409-13. PMC: 431584. DOI: 10.1073/pnas.74.8.3409. View

12.

Paterson B, Bishop J . Changes in the mRNA population of chick myoblasts during myogenesis in vitro. Cell. 1977; 12(3):751-65. DOI: 10.1016/0092-8674(77)90275-6. View

13.

Cavalieri R, Havell E, Vilcek J, Pestka S . Induction and decay of human fibroblast interferon mRNA. Proc Natl Acad Sci U S A. 1977; 74(10):4415-9. PMC: 431953. DOI: 10.1073/pnas.74.10.4415. View

14.

Havell E, Yip Y, Vilcek J . Characteristics of human lymphoblastoid (Namalva) interferon. J Gen Virol. 1978; 38(1):51-9. DOI: 10.1099/0022-1317-38-1-51. View

15.

Sehgal P, Lyles D, Tamm I . Superinduction of human fibroblast interferon production: further evidence for increased stability of interferon mRNA. Virology. 1978; 89(1):186-98. DOI: 10.1016/0042-6822(78)90051-x. View

16.

Havell E, Hayes T, Vilcek J . Synthesis of two distinct interferons by human fibroblasts. Virology. 1978; 89(1):330-4. DOI: 10.1016/0042-6822(78)90068-5. View

17.

Slate D, Ruddle F . Fibroblast interferon in man is coded by two loci on separate chromosomes. Cell. 1979; 16(1):171-80. DOI: 10.1016/0092-8674(79)90198-3. View

18.

Dalton B, Paucker K . Antigenic properties of human lymphoblastoid interferons. Infect Immun. 1979; 23(2):244-8. PMC: 414155. DOI: 10.1128/iai.23.2.244-248.1979. View

19.

Meager A, Graves H, Burke D, Swallow D . Involvement of a gene on chromosome 9 in human fibroblast interferon production. Nature. 1979; 280(5722):493-5. DOI: 10.1038/280493a0. View

20.

Hayes T, Yip Y, Vilcek J . Le interferon production by human fibroblasts. Virology. 1979; 98(2):351-63. DOI: 10.1016/0042-6822(79)90558-0. View