Sequence of the N-terminal Half of Bacillus Amyloliquefaciens Alpha-amylase

Overview

Journal Biochem J

Specialty Biochemistry

Date 1980 Feb 1

PMID 6156671

Citations 2

Authors

H Chung

F FRIEDBERG

Affiliations

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Abstract

Bacillus amyloliquefaciens alpha-amylase (1,4-alpha-D-glucan glucanohydrolase. EC 3.2.1.1), which is commercially supplied as 'Bacillus subtilis alpha-amylase' does not cross-react immunologically with B. subtilis alpha-amylase. This enzyme (from B. amyloliquefaciens) was cleaved by treatment with CNBr into seven fragments. Peptide A was selected for sequence determination. It is the longest one, containing 185 amino acids (i.e. approx. 50% of the total molecule) and connects to the hexapeptide of the N-terminus. Its primary structure was aligned by use of various proteolytic enzymes. The sequence of amino acids 181-184 is identical with that of amino acids 14-17 of the alpha-amylase isolated from B. subtilis (except that amino acid 183 is asparagine rather than aspartic acid).

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References

Tang J, Hartley B . A diagonal electrophoretic method for selective purification of methionine peptides. Biochem J. 1967; 102(2):593-9. PMC: 1270284. DOI: 10.1042/bj1020593. View

WELKER N, Campbell L . Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis. J Bacteriol. 1967; 94(4):1124-30. PMC: 276783. DOI: 10.1128/jb.94.4.1124-1130.1967. View

EDMAN P, Begg G . A protein sequenator. Eur J Biochem. 1967; 1(1):80-91. DOI: 10.1007/978-3-662-25813-2_14. View

Moore S . Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction. J Biol Chem. 1968; 243(23):6281-3. View

Jones R . Automatic peptide chromatography. Methods Biochem Anal. 1970; 18:205-58. DOI: 10.1002/9780470110362.ch4. View

SMITHIES O, Gibson D, Fanning E, Goodfliesh R, Gilman J, Ballantyne D . Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac. Biochemistry. 1971; 10(26):4912-21. DOI: 10.1021/bi00802a013. View

Pisano J, Bronzert T, BREWER Jr H . Advances in the gas chromatographic analysis of amino acid phenyl- and methylthiohydantoins. Anal Biochem. 1972; 45(1):43-59. DOI: 10.1016/0003-2697(72)90006-1. View

Kulbe K . Micropolyamide thin-layer chromatography of phenylthiohydantoin amino acids (PTH) at subnanomolar level. A rapid microtechnique for simultaneous multisample identification after automated Edman degradations. Anal Biochem. 1974; 59(2):564-73. DOI: 10.1016/0003-2697(74)90310-8. View

Heil A, Muller G, NODA L, PINDER T, Schirmer H, Schirmer I . The amino-acid sequence of sarcine adenylate kinase from skeletal muscle. Eur J Biochem. 1974; 43(1):131-44. DOI: 10.1111/j.1432-1033.1974.tb03393.x. View

10.

FRIEDBERG F, Thomsen J . The N-terminal amino acid sequence of Bacillus subtilis alpha-amylase. Acta Chem Scand B. 1974; 28(7):815-6. DOI: 10.3891/acta.chem.scand.28b-0815. View

11.

Bailey G, Gillett D, Hill D, Petersen G . Automated sequencing of insoluble peptides using detergent. Bacteriophage fl coat protein. J Biol Chem. 1977; 252(7):2218-25. View

12.

Hogg R, Hermodson M . Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r. J Biol Chem. 1977; 252(14):5135-41. View

13.

Klapper D, Wilde 3rd C, Capra J . Automated amino acid sequence of small peptides utilizing Polybrene. Anal Biochem. 1978; 85(1):126-31. DOI: 10.1016/0003-2697(78)90282-8. View

14.

Podell D, Abraham G . A technique for the removal of pyroglutamic acid from the amino terminus of proteins using calf liver pyroglutamate amino peptidase. Biochem Biophys Res Commun. 1978; 81(1):176-85. DOI: 10.1016/0006-291x(78)91646-7. View

15.

Mantsala P, ZALKIN H . Membrane-bound and soluble extracellular alpha-amylase from Bacillus subtilis. J Biol Chem. 1979; 254(17):8540-7. View

16.

Detera S, FRIEDBERG F . Molecular weight of B. subtilis alpha-amylase derived from chemical studies. Int J Pept Protein Res. 1979; 14(4):364-72. DOI: 10.1111/j.1399-3011.1979.tb01945.x. View

17.

Gross E, WITKOP B . Nonenzymatic cleavage of peptide bonds: the methionine residues in bovine pancreatic ribonuclease. J Biol Chem. 1962; 237:1856-60. View