Frequencies of Sister-chromatid Exchanges in Relation to Cell Kinetics in Lymphocyte Cultures

Overview

Journal Mutat Res

Publisher Elsevier

Specialty Genetics

Date 1980 May 1

PMID 6155609

Citations 5

Authors

E Giulotto

A Mottura

R Giorgi

L De Carli

F Nuzzo

Affiliations

Soon will be listed here.

Abstract

The frequency of sister-chromatid exchanges (SCE) was determined on second-division metaphases of lymphocytes stimulated by phytohaemagglutinin (PHA) during 9 days of culture. By using either a continuous or a pulsed bromodeoxyuridine (BUdR) treatment, cells were selected that had divided only twice, or at least twice, after different culture periods. No significant differences were observed in the SCE frequencies among the various samples. The incidence of SCE appears to be independent of the proliferation properties of cultured lymphocytes, such as length of cell cycle, fast or delayed response to PHA and number of divisions performed in vitro.

Citing Articles

Increased rate of spontaneous mitotic recombination in T lymphocytes from a Bloom's syndrome patient using a flow-cytometric assay at HLA-A locus.

Kusunoki Y, Hayashi T, Hirai Y, Kushiro J, Tatsumi K, Kurihara T Jpn J Cancer Res. 1994; 85(6):610-8.

PMID: 8063614 PMC: 5919530. DOI: 10.1111/j.1349-7006.1994.tb02403.x.

5-bromodeoxyuridine induced sister chromatid exchange frequencies in primate lymphocytes.

Barnett R, Wallace J Experientia. 1982; 38(5):542-3.

PMID: 7095087 DOI: 10.1007/BF02327039.

Sister chromatid exchange in highly purified human B and T lymphocytes.

Miller K Hum Genet. 1986; 72(2):160-3.

PMID: 3484717 DOI: 10.1007/BF00283937.

Cytokinetics and SCE baselines in rat and human lymphocytes during successive divisions in the presence of different culture media.

Sinha A, Bruce R, Gollapudi B In Vitro Cell Dev Biol. 1988; 24(9):927-30.

PMID: 3170449 DOI: 10.1007/BF02623904.

SCE variability in lymphocytes and fibroblasts. A controlled study.

Lukusa T, Vercauteren P, Van Den Berghe H, Cassiman J Hum Genet. 1988; 80(2):117-23.

PMID: 3169733 DOI: 10.1007/BF00702852.