Alterations of a Mouse Mammary Tumor Virus Glycoprotein with Interferon Treatment

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1980 Apr 1

PMID 6154805

Citations 3

Authors

M J Yagi

N W King Jr

J G BEKESI

Affiliations

Soon will be listed here.

Abstract

The effects of exogenous mouse interferon on the MJY-alpha mammary tumor cell line chronically infected with mouse mammary tumor virus (MMTV) were examined. Interferon at concentrations of 25 to 2,000 IU/ml in culture medium did not alter the growth rate or morphology of the cell layers. Electron microscopic examination of interferon-treated cells indicated a decrease in the numbers of A-type and budding B-type particles of MMTV. However, the levels of extracellular MMTV virions in the culture supernatants were not significantly reduced. Profiles of MMTV glycoproteins and nonglycosylated polypeptides obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of virions purified from interferon-treated cultures revealed increases in the relative levels of the 60,000-dalton glycoprotein, gp60.

Citing Articles

GP60 and SPARC as albumin receptors: key targeted sites for the delivery of antitumor drugs.

Ji Q, Zhu H, Qin Y, Zhang R, Wang L, Zhang E Front Pharmacol. 2024; 15:1329636.

PMID: 38323081 PMC: 10844528. DOI: 10.3389/fphar.2024.1329636.

Accumulation and breakdown of RNA-deficient intracellular virus particles in interferon-treated NIH 3T3 cells chronically producing Moloney murine leukemia virus.

Aboud M, Hassan Y J Virol. 1983; 45(2):489-95.

PMID: 6187933 PMC: 256441. DOI: 10.1128/JVI.45.2.489-495.1983.

Effect of interferon on assembly of intracellular Moloney murine leukemia virus particles in chronically infected 3T3/NIH cells.

Aboud M, Hassan Y, Huleihel M Arch Virol. 1982; 74(4):249-58.

PMID: 6187315 DOI: 10.1007/BF01314158.

References

Brinkley B, Murphy P, Richardson L . Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol. 1967; 35(1):279-83. PMC: 2107120. DOI: 10.1083/jcb.35.1.279. View

Sato T . A modified method for lead staining of thin sections. J Electron Microsc (Tokyo). 1968; 17(2):158-9. View

Tilles J, FINLAND M . Microassay for human and chick cell interferons. Appl Microbiol. 1968; 16(11):1706-7. PMC: 547743. DOI: 10.1128/am.16.11.1706-1707.1968. View

Came P, Moore D . Inhibition of spontaneous mammary carcinoma of mice by treatment with interferon and poly I:C. Proc Soc Exp Biol Med. 1971; 137(1):304-5. DOI: 10.3181/00379727-137-35565. View

Lindahl P, Leary P, GRESSER I . Enhancement by interferon of the specific cytotoxicity of sensitized lymphocytes. Proc Natl Acad Sci U S A. 1972; 69(3):721-5. PMC: 426543. DOI: 10.1073/pnas.69.3.721. View

GRESSER I, Maury C, Brouty-Boye D . Mechanism of the antitumour effect of interferon in mice. Nature. 1972; 239(5368):167-8. DOI: 10.1038/239167a0. View

GRESSER I . Antitumor effects of interferon. Adv Cancer Res. 1972; 16:97-140. DOI: 10.1016/s0065-230x(08)60339-5. View

Compans R . Influenza virus proteins. II. Association with components of the cytoplasm. Virology. 1973; 51(1):56-70. DOI: 10.1016/0042-6822(73)90365-6. View

Chirigos M, PEARSON J . Brief communication: Cure of murine leukemia with drug and interferon treatment. J Natl Cancer Inst. 1973; 51(4):1367-8. DOI: 10.1093/jnci/51.4.1367. View

10.

Yagi M . Cultivation and characterization of BALB-cfC3H mammary tumor cell lines. J Natl Cancer Inst. 1973; 51(6):1849-60. DOI: 10.1093/jnci/51.6.1849. View

11.

Scheid A, Choppin P . Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974; 57(2):475-90. DOI: 10.1016/0042-6822(74)90187-1. View

12.

ANKEL H, CHANY C, Galliot B, Chevalier M, Robert M . Antiviral effect of interferon covalently bound to sepharose. Proc Natl Acad Sci U S A. 1973; 70(8):2360-3. PMC: 433735. DOI: 10.1073/pnas.70.8.2360. View

13.

Yagi M . Further observations on the production of oncornaviruses by MJY-alpha cell line. J Natl Cancer Inst. 1974; 53(5):1383-5. DOI: 10.1093/jnci/53.5.1383. View

14.

Friedman R, Ramseur J . Inhibition of murine leukemia virus production in chronically infected AKR cells: a novel effect of interferon. Proc Natl Acad Sci U S A. 1974; 71(9):3542-4. PMC: 433810. DOI: 10.1073/pnas.71.9.3542. View

15.

Yagi M . Characteristics of mammary tumor cultures from four mouse strains infected with mammary tumor virus. Cancer Res. 1975; 35(2):370-3. View

16.

Billiau A, Edy V, SOBIS H, DE SOMER P . Influence of interferon on virus-particle synthesis in oncornavirus-carrier lines. II. Evidence for a direct effect on particle release. Int J Cancer. 1974; 14(3):335-40. DOI: 10.1002/ijc.2910140306. View

17.

Friedman R, Chang E, Ramseur J, Myers M . Interferon-directed inhibition of chronic murine leukemia virus production in cell cultures: lack of effect on intracellular viral markers. J Virol. 1975; 16(3):569-74. PMC: 354704. DOI: 10.1128/JVI.16.3.569-574.1975. View

18.

Klenk H, Rott R, Orlich M, Blodorn J . Activation of influenza A viruses by trypsin treatment. Virology. 1975; 68(2):426-39. DOI: 10.1016/0042-6822(75)90284-6. View

19.

BEKESI J, Roboz J, Zimmerman E, HOLLAND J . Treatment of spontaneous leukemia in AKR mice with chemotherapy, immunotherapy, or interferon. Cancer Res. 1976; 36(2 pt 2):631-9. View

20.

Pitha P, Rowe W, Oxman M . Effect of interferon on exogenous, endogenous, and chroniv murine leukemia virus infection. Virology. 1976; 70(2):324-38. DOI: 10.1016/0042-6822(76)90275-0. View